E2758

β-Estradiol

BioReagent, powder, suitable for cell culture

• Szinonimák: 1,3,5-Estratriene-3,17β-diol, 17β-Estradiol, 3,17β-Dihydroxy-1,3,5(10)-estratriene, Dihydrofolliculin
• Tapasztalati képlet (Hill-képlet): C₁₈H₂₄O₂
• CAS-szám: 50-28-2
• Molekulatömeg: 272.38
• Beilstein: 1914275
• EC-szám: 200-023-8
• MDL-szám: MFCD00003693
• UNSPSC kód: 12352209
• PubChem Substance ID: 24894483
• NACRES: NA.77

Tulajdonságok

• biológiai forrás: synthetic (organic)
• Minőségi szint: 200
• termékcsalád: BioReagent
• Teszt: ≥98% (HPLC)
• form: powder
• technika/technikák: cell culture | mammalian: suitable; single cell analysis: suitable
• mp: 176-180 °C (lit.)
• oldhatóság: ethanol: 50 mg/mL, clear, colorless
• kiszállítva: ambient
• tárolási hőmérséklet: room temp
• SMILES string: O[C@H]1CC[C@@]2([H])[C@]3([H])CCC4=CC(O)=CC=C4[C@@]3([H])CC[C@@]21C
• InChI: 1S/C18H24O2/c1-18-9-8-14-13-5-3-12(19)10-11(13)2-4-15(14)16(18)6-7-17(18)20/h3,5,10,14-17,19-20H,2,4,6-9H2,1H3/t14-,15-,16+,17+,18+/m1/s1
• Nemzetközi kémiai azonosító kulcs: VOXZDWNPVJITMN-ZBRFXRBCSA-N
• Géninformáció: human ... ESR1(2099) , ESR2(2100) , ESRRA(2101) , ESRRB(2103) , GPER(2852) , SERPINA6(866) ; mouse ... Esr1(13982) , Esr2(13983) , Esrra(26379) ; rat ... Afp(24177) , Ar(24208) , Esr1(24890) , Esr2(25149) , Shbg(24775)

Leírás

Általános leírás

β-Estradiol or estradiol-17 β (E2) is secreted majorly by the large preovulatory follicles in the ovary. In addition, estradiol can be synthesized de novo from cholesterol have androgens synthesized from cholesterol. The aromatization of androgen by enzyme aromatase (P450arom) results in the β-estradiol production.

Alkalmazás

β-Estradiol has been used:

• as a component of Dulbecco′s modified Eagle medium (DMEM) for culturing mammary tumor cells
• as a oestrogenic compound for testing neuroprotective effects
• as a component of medium199 for culturing oocytes

β-Estradiol is used to study cell differentiation and transformations (tumorigenicity).

Biokémiai/fiziológiai hatások

β-Estradiol (E2) is a major premenopausal hormone in women and is essential for spermiogenesis, sperm maturation and motility in men. Cultured bone marrow stem cells (BMSCs) have ability to synthesize E2 and could be exploited for treating estrogen deficiency. It mediates physiological functions through estrogen receptor (ER) α and ERβ.

The major estrogen secreted by the premenopausal ovary. Estrogens direct the development of the female phenotype in embryogenesis and during puberty by regulating gene transcription and, thus, protein synthesis. It also induces the production of gonadotropins which, in turn, induce ovulation. Exposure to estradiol increases breast cancer incidence and proliferation.

Fizikai forma

powder-RT; stock-frozen in working aliquots, avoid repeated freeze/thaw

Feloldás

To prepare a 20 μg/ml stock solution, add 1 ml absolute ethanol to 1 mg β-estradiol; gently swirl to dissolve; add 49 ml sterile medium while mixing.

Biztonsági adatok

• Piktogramok: GHS08,GHS09
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H351,H360FD,H362,H410
• Óvintézkedésre vonatkozó mondatok: P202 - P260 - P263 - P264 - P273 - P308 + P313
• Veszélyességi osztályok: Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Lact. - Repr. 1A
• Tárolási osztály kódja: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type P3 (EN 143) respirator cartridges