Ugrás a tartalomra
Merck
Összes fotó(1)

Key Documents

View All Documentation

E1270

Sigma-Aldrich

ECM Gel from Engelbreth-Holm-Swarm murine sarcoma

liquid, BioReagent, suitable for cell culture

Szinonimák:

Basement membrane extract (BME), EHS matrix

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

MDL-szám:
MFCD00283131
UNSPSC kód:
12352207
NACRES:
NA.75

termékcsalád

BioReagent

Minőségi szint

300

form

liquid

koncentráció

8-12 mg/mL

technika/technikák

cell culture | mammalian: suitable

UniProt elérési szám

P02468
P02469
P10493
P19137
P43407
Q61001
Q61092
Q61789

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Géninformáció

mouse ... Lama1(16772) , Lama3(16774) , Lama5(16776) , Lamb1-1(16777) , Lamc1(226519) , Lamc2(16782) , Nid1(18073) , Sdc2(15529)

Alkalmazás

ECM gel can used with epithelial cells, endothelial cells, muscle cells, nerve cells and tumor cells.

Biokémiai/fiziológiai hatások

The ECM gel will undergo thermally activated polymerization when brought to 20-40°C, forming a reconstituted basement membrane. PC12 cells will show neurite formation within 2 days when they are grown on a thin layer of this ECM gel.

Every mouse colony used for the production of this product is routinely screened for several pathogens. Tested and found negative for LDEV.

Komponensek

ECM was prepared to a protein concentration of 8-12 mg/mL (in DMEM), containing laminin as a major component, collagen type IV, heparin sulfate proteoglycan, entactin, and other minor components. Addition of collagen type IV to the ECM gel increases polymerization.

Product contains 50 mg/L Gentamicin Sulfate.

Vigyázat

For long term storage, keep product at -20°C. The ECM gel may be stored at 2-8°C for up to 72 hours.

Elkészítési megjegyzés

This product is prepared from Engelbreth-Holm-Swarm sarcoma in mice; dialyzed against chloroform. Gel should be thawed overnight at 2-8°C before use and dispensed to a multiwall plate using a plate and pipettes that are pre-cooled to 2-8°C. The gel may also be diluted up to twofold with 2-8°C Dulbecco′s Modified Eagle′s Medium, and should be done before gel is added to the plate. The product will gel within 5 minutes at 20°C. For prolonged manipulations, work should be conducted below 10°C. Cells can be plated on top of a thin gel layer of 0.5 mm or cultured inside a 1 mm layer. If you culture cells inside, add the cells to the gel prior to plating at a recommended density of 3-4 x 104 cells per mL. To dissociate cells from this gel, use protease in PBS without calcium, magnesium, or EDTA, at a concentration of 0.6-2.4 units per mL.

gyakran vásárolják ezzel a termékkel együtt

Product No.
Leírás
Árazás

Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Lutian Yao et al.
Cytokine, 89, 194-200 (2016-02-13)
Pancreatic cancer is characterized as inflammatory malignancy with a dismal prognosis. There is abundant intratumoral infiltration of macrophages, and most of these tumor associated macrophages (TAM) are induced to be M2 phenotype. The M2 polarized TAM has been demonstrated to
Eva Lichtenegger et al.
Human gene therapy, 30(1), 44-56 (2018-06-20)
Muscle-invasive bladder cancer represents approximately 25% of diagnosed bladder cancer cases and carries a significant risk of death. Oncolytic viruses are novel antitumor agents with the ability to selectively replicate and lyse tumor cells while sparing healthy tissue. We explored
Ievgenia Pastushenko et al.
Nature, 556(7702), 463-468 (2018-04-20)
In cancer, the epithelial-to-mesenchymal transition (EMT) is associated with tumour stemness, metastasis and resistance to therapy. It has recently been proposed that, rather than being a binary process, EMT occurs through distinct intermediate states. However, there is no direct in
Jesper Just et al.
Scientific reports, 10(1), 5835-5835 (2020-04-05)
Ischemic exercise conducted as low-load blood flow restricted resistance exercise (BFRE) can lead to muscle remodelling and promote muscle growth, possibly through activation of muscle precursor cells. Cell activation can be triggered by blood borne extracellular vesicles (EVs) as these
Mallory E Walters et al.
Antioxidants (Basel, Switzerland), 9(6) (2020-07-02)
The aim of this work was to determine the physicochemical and biological activities of hydrolyzed proteins from sonicated oat brans. In addition to the control bran sample, two types of pre-treatment procedures-namely, ultrasonic bath and probe-type sonication-were performed to extract
View All Related Papers

Cikkek

ECM Gel Matrix: Protocols Using EHS Basement Membrane Extracts

Cell culture protocols using ECM Gel Matrix EHS basement membrane extract (BME) for neurite outgrowth, cell invasion and angiogenesis tube formation assays.

Angiogenesis Assays

Cell based angiogenesis assays to analyze new blood vessel formation for applications of cancer research, tissue regeneration and vascular biology.

Controlled Fabrication Methods for Tissue Engineering Constructs

Highlighting existing and novel fabrication methods for both, solid and hydrogel-based scaffold for tissue engineering applications.

Attachment Factors for Three-dimensional Cell Culture

The extracellular matrix (ECM) and its attachment factor components are discussed in this article in relation to their function in structural biology and their availability for in vitro applications.

Összes mutatása

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással