TM4

88111401, mouse testis, Not specified

• UNSPSC kód: 41106514

Tulajdonságok

• product name: TM4, 88111401
• biológiai forrás: mouse testis
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Not specified
• termékek: Transferrin, H-Y Ag, novel retinol-binding protein
• receptorok: Androgen, oestrogen and progesterone receptors
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Mouse BALB/c testis sertoli cell

Sejtvonal leírása

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Alkalmazás

Hormone studies

Táptalaj

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Egyéb megjegyzések

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