CV-1

87032605, African green monkey kidney, Fibroblast

• UNSPSC kód: 41106514
• biológiai forrás: African green monkey kidney
• növekedési mód: Adherent
• kariotípus: 2n = 60, pseudodiploid
• morfológia: Fibroblast
• receptorok: Not specified

Tulajdonságok

• Terméknév: CV-1, from African green monkey kidney, 87032605, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• biológiai forrás: African green monkey kidney
• növekedési mód: Adherent
• kariotípus: 2n = 60, pseudodiploid
• morfológia: Fibroblast
• receptorok: Not specified

Leírás

Sejtvonal eredete

Monkey African Green kidney fibroblast

Sejtvonal leírása

Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.

Táptalaj

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37 °C.

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