BeWo Cell Line human

86082803, human placenta, Epithelial

• Szinonimák: Be-Wo Cells
• UNSPSC kód: 41106514
• biológiai forrás: human placenta
• növekedési mód: Adherent
• kariotípus: modal number =86 range 71-178
• morfológia: Epithelial
• termékek: Gonadotrophin, lactogen and steroid hormones
• receptorok: Not specified
• vonatkozó betegség(ek): cancer

Tulajdonságok

• Terméknév: BeWo Cell Line human, 86082803
• biológiai forrás: human placenta
• leírás: Human choriocarcinoma
• növekedési mód: Adherent
• kariotípus: modal number =86 range 71-178
• morfológia: Epithelial
• termékek: Gonadotrophin, lactogen and steroid hormones
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human choriocarcinoma

Sejtvonal leírása

The first human, trophoblastic endocrine cell type to be maintained in continuous culture. It was initiated from a malignant gestational choriocarcinoma of the foetal placenta. The cells secrete placental hormones including chorionic gonadotrophin (hCG), polypeptide hormones, lactogen, estrogenic and progestational steroids, estrone, estradiol, estriol and progesterone. A high rate of hCG Synthesis has been shown. Cellular differentiation of this stem cell type occurs when hormonal precursors are added to the medium. Due to the high glycolytic activity the cells have to be sub-cultured or supplied with fresh media every 24-48 hours to prevent glucose exhaustion. BeWo has the same DNA profile as JEG3 (Sigma catalogue number 92120308); JEG3 was established by serial cloning of BeWo.

Alkalmazás

Hormone studies

Human BeWo cell line has been used to study the effects of Δ-9-tetrahydrocannabinol (Δ9-THC) on the proliferation and gene transcription in the BeWo trophoblast cell line. It has also been used to study placental transfer of highly purified non-dioxin-like polychlorinated biphenyls in BeWo cells.

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 9,11
D16S539: 13,14
D5S818: 10,11
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 16

Táptalaj

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C; medium change or subculture every 24-48 hours necessary.

Egyéb megjegyzések

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.