PPB023

Tris buffer

Pre-set, pH 8.1, pHast Pack^™, powder

• Szinonimák: 0.1M Tris
• UNSPSC kód: 12161700
• NACRES: NA.25

Tulajdonságok

• Minőségi szint: 100
• termékcsalád: pHast Pack^™
• Forma: powder
• tárolási körülmény: (Tightly closed. Dry)
• szennyeződések: ≤5 ppm Heavy Metals (lead)
• szín: white
• pH: 8.1
• kation nyomok: Fe: ≤10 ppm
• idegen aktivitás: DNase, none detected; Endonuclease, none detected; Exonuclease, none detected; NICKase, none detected; Protease, none detected; RNase, none detected
• tárolási hőmérséklet: room temp

Leírás

Általános leírás

Tris buffer, pH 8.1 is a general biological buffer commonly used in biochemistry and molecular biology. It is isotonic and non-toxic in nature due to the presence of tris base. Tris has a buffering capacity range of pH 7.0 - 9.0, with a pKa of 8.1 at 25°C.

Alkalmazás

Intended for use in:

• Cell culture (sterile filter recommended)
• Western blot
• Protein electrophoresis
• DNA agarose electrophoresis as a component to make Tris acetate-EDTA (TAE) and Tris borate-EDTA (TBE) buffers

Tulajdonságok és előnyök

• Ready-to-use for quick buffer prep
• No measuring and adjusting pH
• Saves time and minimizes effort in the lab
• Biological tests: free of DNase, RNase, protease, and nickase
• Chemical tests: Iron ≤10 ppm, lead ≤5 ppm

Kiszerelés

Foil pouches

Feloldás

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 0.1M Tris solution, pH 8.1 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free. This product is suitable for use in cell culture and electrophoresis applications.

Tárolás és stabilitás

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Egyéb megjegyzések

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Jogi információk

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1