E9379

Stains-All

~95% purity, powder

• Szinonimák: 1-Ethyl-2-[3-(1-ethylnaphtho[1,2-d]thiazolin-2-ylidene)-2-methylpropenyl]naphtho[1,2-d]thiazolium bromide, 3,3′-Diethyl-9-methyl-4,5,4′,5′-dibenzothiacarbocyanine
• Tapasztalati képlet (Hill-képlet): C₃₀H₂₇BrN₂S₂
• CAS-szám: 7423-31-6
• Molekulatömeg: 559.58
• Beilstein: 3865213
• EC-szám: 231-047-7
• MDL-szám: MFCD00078788
• UNSPSC kód: 12171500
• PubChem Substance ID: 24894694
• NACRES: NA.47

Tulajdonságok

• Terméknév: Stains-All, ~95%
• Teszt: ~95%
• Minőségi szint: 200
• Forma: powder
• technika/technikák: microbe id | staining: suitable
• oldhatóság: chloroform: 40 mg (PLUS 4.0 ml)
• alkalmazás(ok): diagnostic assay manufacturing; hematology; histology
• tárolási hőmérséklet: 2-8°C
• SMILES string: [Br-].CCN1C(/Sc2ccc3ccccc3c12)=C\C(C)=C\c4sc5ccc6ccccc6c5[n+]4CC
• InChI: 1S/C30H27N2S2.BrH/c1-4-31-27(33-25-16-14-21-10-6-8-12-23(21)29(25)31)18-20(3)19-28-32(5-2)30-24-13-9-7-11-22(24)15-17-26(30)34-28;/h6-19H,4-5H2,1-3H3;1H/q+1;/p-1
• Nemzetközi kémiai azonosító kulcs: MPBRYMWMMKKRGC-UHFFFAOYSA-M

Leírás

Alkalmazás

Stains-All has been used for the staining of gel made for size determination of hyaluronan. It has also been used for the staining of DNA oligonucleotides separated by nondenaturing polyacrylamide gel electrophoresis.

Biokémiai/fiziológiai hatások

Stains-All is a cationic carbocyanine dye and is a multipurpose dye. It stains sialoglycoproteins blue, Ca²⁺ binding proteins deep blue to violet, proteins red and lipids yellow-orange. It also stains RNA (bluish purple) and DNA (blue).

As little as 3 ng (123 BP fragment) of pBR322/Hae III digest DNA and 90 ng tRNA can be detected on a polyacrylamide gel. PAGE gels are stained in the dark and then destaining by removing the gel from the staining solution and exposing it to the light from a light box until sufficient destaining has occurred. A staining solution is typically made by dissolving the dye in formamide and buffer.

Vigyázat

Upon continued exposure to light the nucleic acid-dye complex will fade and disappear.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)