A9056

Bovine Serum Albumin

heat shock fraction, microbiologically tested, ≥96% (agarose gel electrophoresis)

• Szinonimák: Albumin bovine serum, BSA, Bovine albumin
• CAS-szám: 9048-46-8
• EC-szám: 232-936-2
• MDL-szám: MFCD00130384
• UNSPSC kód: 12352202
• NACRES: NA.27

Tulajdonságok

• biológiai forrás: bovine
• Minőségi szint: 200
• Teszt: ≥96% (agarose gel electrophoresis)
• Forma: lyophilized powder
• molekulatömeg: ~66 kDa
• tisztítva: heat shock fractionation
• kiszerelés: poly bottle of
• eredet: USA origin
• technika/technikák: cell culture | mammalian: suitable
• szennyeződések: ≤5.0% Loss on drying
• veszteség: ≤5%
• pH: 7
• oldhatóság: water: soluble (40 mg/ml)
• UniProt elérési szám: P02769
• idegen aktivitás: BT virus, none detected; Microbiological test (pass); VSV virus, none detected; growth of Borrelia burgdorferi in BSK II medium (Suitable for)
• tárolási hőmérséklet: 2-8°C
• Géninformáció: bovine ... ALB(280717)

Leírás

Általános leírás

Albumin is the most abundant plasma protein in humans. High levels of albumin are associated with dehydration. Albumin turnover is seen in infants with iron deficiency anemia. Serum albumin is a reliable prognostic indicator for liver disease. Oxidative damage of albumin is associated with advanced liver disease.

Alkalmazás

Bovine Serum Albumin has been used as a supplement in Dulbecco′s modified eagle medium (DMEM). It has also been used in immunostaining of cell arrays.

Biokémiai/fiziológiai hatások

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Alkalmasság

Suitable for growth of Borrelia burgdorferi in BSK II medium

Elkészítési megjegyzés

Prepared using heat shock fractionation

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)