Összes fotó(1)
Fontos dokumentumok
10280879001
Roche
5-Bromo-2′-deoxyuridine
>97%, crystalline solid, pkg of 1 g
Szinonimák:
5-Bromo-2′-deoxyuridine, brdu, 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Tapasztalati képlet (Hill-képlet):
C9H11BrN2O5
CAS-szám:
Molekulatömeg:
307.10
Beilstein:
30395
MDL-szám:
UNSPSC kód:
12352204
PubChem Substance ID:
Javasolt termékek
Minőségi szint
Teszt
>97%
Forma
crystalline solid
molekulatömeg
307.1
kiszerelés
pkg of 1 g
gyártó/kereskedő neve
Roche
mp
191-194 °C (dec.) (lit.)
kiszállítva
wet ice
tárolási hőmérséklet
2-8°C
SMILES string
OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI
1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
Nemzetközi kémiai azonosító kulcs
WOVKYSAHUYNSMH-RRKCRQDMSA-N
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Általános leírás
It is conventionally used as a marker for S-phase synthesis of DNA.
Alkalmazás
5-Bromo-2′-deoxyuridine has been used to label cells in the S-phase of the cell cycle and primary hepatocytes during cell proliferation studies.
Immunochemically detectable nucleotide that can be incorporated into DNA instead of thymidine. Used for in vivo studies of DNA synthesis and for the detection of mutagenic substances, according to the sister chromatid exchange (SCE) method.
Biokémiai/fiziológiai hatások
Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.
Minőség
Purity: 97% (from N), chromatographically homogeneous
Elkészítési megjegyzés
Working concentration: According to literature for cell culture, a final concentration of 10 μmol/l has been used.
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
According to literature, 5 to 50 mg/kg body weight mice have been used for the detection of cell proliferation.
Preparation of Working Solution
Corresponding to the BrdU-labeling reagent which is included in the Roche Cell Proliferation ELISA, BrdU kits and in the Roche 5-Bromo-2′-deoxy-uridine Labeling and Detection Kits, prepare the BrdU working solution by dissolving the substance in PBS to a 10 mM stock solution (MW of BrdU = 307.1 D). For the in vivo use of BrdU dissolve in PBS; for the in vitro use of BrdU, dissolve in double-distilled water at the same 10 mM concentration.
Storage conditions (working solution): -15 to -25 °C
Egyéb megjegyzések
For life science research only. Not for use in diagnostic procedures.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Muta. 1B - Repr. 2
Tárolási osztály kódja
13 - Non Combustible Solids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
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