Ugrás a tartalomra
Merck
Összes fotó(1)

Key Documents

UC0E03

Sigma-Aldrich

UCOE® Dual Expression Puromycin Vector Set

Szinonimák:

UCOE Mammalian Protein Expression Vectors, UCOE Mammalian Protein Expression Plasmids, Ubiquitous Chromatin Opening Element, UCOE03

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
41106621
eCl@ss:
34360190
NACRES:
NA.51

kiszállítva

dry ice

Minőségi szint

100

Általános leírás

The Ubiquitous Chromatin Opening Element (UCOE) technology offers significant advances in recombinant mammalian protein expression. Unlike traditional vectors, UCOE-containing vectors have the capacity to ease the process of isolating cell line clones that express high-levels of recombinant proteins. UCOE sequences promote reproducible and stable high level expression of a linked gene by altering chromatin structure to a transcriptionally permissive state that negates epigenetic-mediated (e.g. DNA methylation) silencing.

CET 1019 HD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a strong human cytomegalovirus (hCMV) immediate early promoter-enhancer element.

CET 1019 AD-puro-SceI is a mammalian expression plasmid vector containing both the mouse Rps3 UCOE and the human HNRPA2B1-CBX3 UCOE each upstream of a guinea pig CMV (gpCMV) promoter-enhancer element.

The CET 1019 HD-puro-SceI and CET 1019 AD-puro-SceI vectors possess the following features:

  • A multiple cloning site downstream of the UCOE-CMV combinations containing unique restriction enzyme sites to permit easy insertion of any gene of interest (cDNA or genomic)
  • A polyadenylation element from the SV40 early region located downstream of the multiple cloning site for appropriate mRNA 3 end formation
  • A puromycin antibiotic resistance gene for selection of stably transfected mammalian cells
  • A β-lactamase gene for plasmid selection and propagation in transformed prokaryotic cells in the presence of ampicillin
  • An I-SceI homing restriction endonuclease site for linearization of constructs prior to transfection into mammalian cells, which favors integration into the target cell genome via the pre-generated free DNA ends and thus retains the integrity of the UCOE-CMV-transgene cassettes


THIS PRODUCT IS ONLY AVAILABLE FOR SALE TO ACADEMIC INSTITUTIONS OR NOT-FOR-PROFIT ENTITIES FOR USE UNDER THIS PRODUCT LABEL LICENSE. FOR INFORMATION ON COMMERCIAL LICENSING OF THE PATENTED UCOE TECHNOLOGY, PLEASE CONTACT licensing@emdmillipore.com.

Komponensek

1) 10ug CET 1019 HD-puro-SceI (+) Vector (CS221292)

2) 10ug CET 1019 AD-puro-SceI (+) Vector (CS221293))

3) 10ug DC HD-puro (-) Vector (CS221283)

4) 10ug DC AD-puro (-) Vector (CS221295)

Egyéb megjegyzések

Concentration: Please refer to lot specific datasheet.

Jogi információk

UCOE is a registered trademark of Merck KGaA, Darmstadt, Germany

Tárolási osztály kódja

12 - Non Combustible Liquids

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Michael Antoniou et al.
Genomics, 82(3), 269-279 (2003-08-09)
The genetic elements that are responsible for establishing a transcriptionally competent, open chromatin structure at a region of the genome that consists only of ubiquitously expressed, housekeeping genes are currently unknown. We demonstrate for the first time through functional analysis
Fang Zhang et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 18(9), 1640-1649 (2010-07-01)
DNA methylation may restrict the activity of gene transfer vectors due to inadvertent silencing. In P19 embryonic carcinoma cells in vitro, we found that transgene expression regulated by the SFFV LTR and EF1 alpha promoter declined rapidly within 16 days

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

Lépjen kapcsolatba a szaktanácsadással