QIA39
FragEL DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme
Szinonimák:
TUNEL Assay
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41116133
NACRES:
NA.51
Javasolt termékek
használat
sufficient for 50 tests
Minőségi szint
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Calbiochem®
tárolási körülmény
OK to freeze
avoid repeated freeze/thaw cycles
technika/technikák
flow cytometry: suitable
bemenet
sample type cell suspension(s)
sample type frozen section(s)
sample type paraffin section(s)
sample type fixed-cell preparation(s)
észlelési módszer
fluorometric
kiszállítva
wet ice
tárolási hőmérséklet
−20°C
Általános leírás
Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.
Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.
Komponensek
Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.
Figyelmeztetés
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Specifikációk
Assay Time: 2 h
Elv
The Calbiochem Fluorescein-FragEL DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.
Tárolás és stabilitás
Fluorescein-FragEL kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).
Egyéb megjegyzések
Darzynkiewicz, Z., et al. 1997. Cytometry27, 1.
Frey, T. 1997. Cytometry28, 253.
Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
Gavrieli, Y., et al. 1992. J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol.65, 437.
Martin, S.J., et al. 1990. J. Immunology145, 1859.
Wyllie, A. H. 1980. Nature284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.
Frey, T. 1997. Cytometry28, 253.
Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992. Cytometry13, 795.
Gavrieli, Y., et al. 1992. J. Cell Biol.119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol.65, 437.
Martin, S.J., et al. 1990. J. Immunology145, 1859.
Wyllie, A. H. 1980. Nature284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer26, 239.
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Jogi információk
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Parafilm is a registered trademark of Bemis Company, Inc.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B - Eye Irrit. 2
Tárolási osztály kódja
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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