MABF3094
Anti-BAFF/CD257 Antibody, clone 5A8
Szinonimák:
B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13B
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rat
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
5A8, monoclonal
faj reaktivitás
mouse
kiszerelés
antibody small pack of 100
technika/technikák
activity assay: suitable
direct ELISA: suitable
western blot: suitable
izotípus
IgG1κ
epitóp szekvencia
Unknown
Protein ID elérési szám
UniProt elérési szám
Géninformáció
mouse ... Klrk1(24099)
Egyediség
Clone 5A8 is a rat monoclonal antibody that detects B-cell activating factor (BAFF/CD257).
Immunogén
Rat RBL cells expressing full-length mouse BAFF.
Alkalmazás
Quality Control Testing
Evaluated by Western Blotting with recombinant murine BAFF protein .
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .
Tested Applications
Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).
ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting with recombinant murine BAFF protein .
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .
Tested Applications
Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).
ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Cél megnevezése
Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Kowalczyk-Quintas, C., et al (2016). J. Biol. Chem. 291(38); 19826-19834; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).
Fizikai forma
Purified rat monoclonal antibody IgG1 in PBS without azide.
Feloldás
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Tárolás és stabilitás
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással