MABE647
Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172
clone RM172, from rabbit
Szinonimák:
Histone H3.3, Histone H3
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
RM172, monoclonal
faj reaktivitás
human
technika/technikák
multiplexing: suitable
western blot: suitable
izotípus
IgG
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
acetylation (Lys27)
Géninformáció
human ... H3F3B(3021)
Általános leírás
Histone H3, also known as Histone H3.1t (H3/t), H3t, H3/g, and encoded by the gene name HIST3H3/ H3FT, is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Therefore, histones play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. High levels of phosphorylation of Histone H3 are associated with mitosis.
Egyediség
This antibody reacts to Histone H3 acetylated at Lysine 27 (K27ac). No cross reactivity with other acetylated Lysines in Histone H3.
Immunogén
A linear peptide corresponding to human Histone H3 acetylated at Lys27.
Alkalmazás
Multiplex Analysis (Mplex): 0.1-0.5 μg/mL from a representative lot was used in Multiplex.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
This Anti-acetyl-Histone H3 (Lys27) Antibody, clone RM172 is validated for use in Western Blotting, Multiplexing for the detection of acetyl-Histone H3.
Minőség
Evaluated by Western Blotting in untreated and sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected acetyl-Histone H3 (Lys27) in sodium butyrate treated HeLa acid extract.
Western Blotting Analysis (WB): 1 μg/mL of this antibody detected acetyl-Histone H3 (Lys27) in sodium butyrate treated HeLa acid extract.
Cél megnevezése
~17 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Fizikai forma
Format: Purified
Protein A purified
Rabbit monoclonal in PBS with 1% BSA and 0.09% sodium azide
Tárolás és stabilitás
Stable for 1 year at 2-8°C from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Egyéb megjegyzések
Concentration: Please refer to lot specific datasheet.
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
does not flash
Lobbanási pont (C)
does not flash
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Michael S Hoetker et al.
Nature cell biology, 25(8), 1121-1134 (2023-07-18)
The epigenetic mechanisms that maintain differentiated cell states remain incompletely understood. Here we employed histone mutants to uncover a crucial role for H3K36 methylation in the maintenance of cell identities across diverse developmental contexts. Focusing on the experimental induction of
Chi Sun et al.
Scientific reports, 13(1), 12899-12899 (2023-08-10)
Enhancers function with a basal promoter to control the transcription of target genes. Enhancer regulatory activity is often studied using reporter-based transgene assays. However, unmatched results have been reported when selected enhancers are silenced in situ. In this study, using
Steven J Wu et al.
Nature biotechnology, 39(7), 819-824 (2021-04-14)
Methods for quantifying gene expression1 and chromatin accessibility2 in single cells are well established, but single-cell analysis of chromatin regions with specific histone modifications has been technically challenging. In this study, we adapted the CUT&Tag method3 to scalable nanowell and
Steven Henikoff et al.
eLife, 9 (2020-11-17)
Chromatin accessibility mapping is a powerful approach to identify potential regulatory elements. A popular example is ATAC-seq, whereby Tn5 transposase inserts sequencing adapters into accessible DNA ('tagmentation'). CUT&Tag is a tagmentation-based epigenomic profiling method in which antibody tethering of Tn5
Julien Jarroux et al.
EMBO reports, 22(7), e50193-e50193 (2021-05-08)
Epithelial-to-mesenchymal transition (EMT) describes the loss of epithelial traits and gain of mesenchymal traits by normal cells during development and by neoplastic cells during cancer metastasis. The long noncoding RNA HOTAIR triggers EMT, in part by serving as a scaffold
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással