MAB5356
Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
clone Rho 1D4, Chemicon®, from mouse
Szinonimák:
Anti-CSNBAD1, Anti-OPN2, Anti-RP4
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(5)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
Rho 1D4, monoclonal
application:
ICC
IHC
IP
WB
IHC
IP
WB
faj reaktivitás:
vertebrates
technika/technikák:
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
105
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
Rho 1D4, monoclonal
faj reaktivitás
vertebrates
gyártó/kereskedő neve
Chemicon®
technika/technikák
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG1
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... RHO(6010)
Egyediség
Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.
Immunogén
Bovine rhodopsin
Epitope: C-terminus, last 9 amino acids
Alkalmazás
Anti-Rhodopsin Antibody, C-terminus, last 9 amino acids, clone Rho 1D4 detects level of Rhodopsin & has been published & validated for use in IC, IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western blot: 1:100-1:500 using ECL. Suggested dilution buffer is PBS and blocking buffer is PBS containing 5% milk. Recognizes 36 kDa monomers, dimmers and trimers depending on sample preparation. Boiling induces the aggregation of rhodopsin.
Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.
Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.
Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Cél megnevezése
48 kDa
Fizikai forma
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Tárolás és stabilitás
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analízis megjegyzés
Control
Eye
Eye
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
M Keith Wyatt et al.
PloS one, 8(6), e68088-e68088 (2013-07-11)
Age-related macular degeneration (AMD) is a major cause of vision loss. It is associated with development of characteristic plaque-like deposits (soft drusen) in Bruch's membrane basal to the retinal pigment epithelium (RPE). A sequence variant (Y402H) in short consensus repeat
Bernice H Wong et al.
The Journal of biological chemistry, 291(20), 10501-10514 (2016-03-25)
Eye photoreceptor membrane discs in outer rod segments are highly enriched in the visual pigment rhodopsin and the ω-3 fatty acid docosahexaenoic acid (DHA). The eye acquires DHA from blood, but transporters for DHA uptake across the blood-retinal barrier or
Tomomi Sakai et al.
Journal of autoimmunity, 81, 13-23 (2017-03-23)
TREX1/DNASE III, the most abundant 3'-5' DNA exonuclease in mammalian cells, is tail-anchored on the endoplasmic reticulum (ER). Mutations at the N-terminus affecting TREX1 DNase activity are associated with autoimmune and inflammatory conditions such as Aicardi-Goutières syndrome (AGS). Mutations in
Laurie L Molday et al.
Methods in molecular biology (Clifton, N.J.), 1177, 1-15 (2014-06-20)
Incorporation of short epitope tags into proteins for recognition by commercially available monoclonal or polyclonal antibodies has greatly facilitated the detection, characterization, localization, and purification of heterologously expressed proteins for structure-function studies. A number of tags have been developed, but
Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis.
Burstyn-Cohen, T; Lew, ED; Traves, PG; Burrola, PG; Hash, JC; Lemke, G
Neuron null
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