MAB424R
Anti-PCNA Antibody, clone PC10
clone PC10, Chemicon®, from mouse
Szinonimák:
Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
PC10, monoclonal
application:
FACS
IHC
IP
WB
IHC
IP
WB
faj reaktivitás:
invertebrates, vertebrates
technika/technikák:
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
citations:
19
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
PC10, monoclonal
faj reaktivitás
invertebrates, vertebrates
gyártó/kereskedő neve
Chemicon®
technika/technikák
flow cytometry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable
izotípus
IgG2a
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... PCNA(5111)
Related Categories
Általános leírás
Proliferating cell nuclear antigen (PCNA) is a 36 kDa molecule that is highly conserved between species. PCNA was first identified as the antigen for a subpopulation of autoantibodies in patients with systemic lupus erythematosus (Miyachi, 1978; Takasaki, 1984; Ogata, 1987). It has since been determined that PCNA serves as a co-factor for DNA polymerase delta in S-phase as well as during DNA synthesis associated with mechanisms involved in DNA damage repair (Tan, 1987; Bravo, 1987). The temporal specificity of PCNA expression makes it an ideal marker for cell proliferation. PCNA begins to accumulate during the G1 phase of the cell cycle, is most abundant during the S phase, and declines during the G2/M phase (Kurki, 1988). Since the half-life of PCNA exceeds 20 hours, it may be possible to detect the protein in non-cycling cells.
Egyediség
Clone PC10 recognizes PCNA from all vertebrate and insect species tested and has been used to identify transformed cells (Kurki, 1988), proliferating cells in solid tumors (Smetana, 1983), and blast cells in leukemia patients (Takasaki, 1984). Immunohistochemistry with clone PC10 has been used to study the expression of PCNA in paraffin sections of normal tissues and lymphoid neoplasms (Hall, 1990). In proliferating cells, PCNA staining pattern is predominantly nuclear. By western blot, the antibody detects a polypeptide migrating at 36 kDa with an isoelectric point of 4.8.
Immunogén
Rat PCNA made in the protein A vector pR1T2T
Alkalmazás
Anti-PCNA Antibody, clone PC10 is a mouse monoclonal antibody for detection of PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor & has been validated in FC, IP, WB,IHC(P).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western blot: 1:250-1:1000. Nuclear extracts are preferred over whole cell protein extracts.
Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin′s reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.
Immunoprecipitation
Indirect Flow Cytometry
Optimal working dilutions must be determined by the end user.
Immunohistochemistry: 1:20-1:200. Recommended for paraffin embedded tissue sections only. May be used on material fixed in a wide range of fixatives including formalin (buffered and unbuffered), methacarn and Bouin′s reagent. The time of fixation can markedly affect the intensity of PCNA immunoreactivity. Staining is reduced (and may be abolished) if sections are baked onto glass slides. Air-drying overnight on poly-L-lysine coated slides is recommended. 60 minute incubation at 25°C with standard ABC technique is recommended.
Immunoprecipitation
Indirect Flow Cytometry
Optimal working dilutions must be determined by the end user.
Fizikai forma
Format: Purified
Purified immunoglobulin (ion-exchange chromatography). Liquid.
Tárolás és stabilitás
Maintain at 2 to 8°C for up to 6 months after date of receipt.
Analízis megjegyzés
Control
Tonsil or reactive lymph node tissue
Tonsil or reactive lymph node tissue
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
12 - Non Combustible Liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
R Choudhury et al.
Scientific reports, 11(1), 4213-4213 (2021-02-20)
Early-life gut microbial colonisation is known to influence host physiology and development, shaping its phenotype. The developing gastro-intestinal tract of neonatal piglets provides a "window of opportunity" for programming their intestinal microbiota composition and corresponding intestinal development. Here, we investigated
Mark W Zimmerman et al.
Science advances, 7(43), eabe0834-eabe0834 (2021-10-21)
[Figure: see text].
Ronja Balhorn et al.
International journal of molecular sciences, 21(13) (2020-07-08)
Hypertensive patients have an increased risk of developing chronic kidney disease (CKD). Many of these patients have increased levels of the blood pressure regulating mineralocorticoid aldosterone. As a protection against aldosterone-induced damage, kidney cells can upregulate key regulators of the
Raka Choudhury et al.
Frontiers in immunology, 14, 1208891-1208891 (2023-06-12)
Diet-microbiota-host interactions are increasingly studied to comprehend their implications in host metabolism and overall health. Keeping in mind the importance of early life programming in shaping intestinal mucosal development, the pre-weaning period can be utilised to understand these interactions in
David Sarrio et al.
Frontiers in cell and developmental biology, 10, 813929-813929 (2022-03-15)
Gasdermins (GSDM) genes play complex roles in inflammatory diseases and cancer. Gasdermin-B (GSDMB) is frequently upregulated in human cancers, especially in HER2-amplified breast carcinomas, and can promote diverse pro-tumor functions (invasion, metastasis, therapy-resistance). In particular, the GSDMB shortest translated variant
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