MAB4100
Anti-MRP1 Antibody, clone QCRL-1
clone QCRL-1, Chemicon®, from mouse
Szinonimák:
Multidrug Resistance Protein-1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
QCRL-1, monoclonal
application:
FACS
ICC
IHC (p)
WB
ICC
IHC (p)
WB
faj reaktivitás:
human
technika/technikák:
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
citations:
16
Javasolt termékek
biológiai forrás
mouse
Minőségi szint
antitest forma
purified immunoglobulin
antitest terméktípus
primary antibodies
klón
QCRL-1, monoclonal
faj reaktivitás
human
gyártó/kereskedő neve
Chemicon®
technika/technikák
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
izotípus
IgG1
UniProt elérési szám
kiszállítva
wet ice
Géninformáció
human ... ABCC1(4363)
Related Categories
Egyediség
Reacts with a defined linear epitope (aa 918-924) of MRP1, a 190 kDa transmembrane
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
phosphoglycoprotein overexpressed in various human multidrug resistant tumor cell lines
and tumors (Cole et al., 1992; Hipfner et al., 1994, 1996, 1999; Wright et al, 1998). Does not cross react with the mouse mrp1, or human MDR1, MDR3,
MRP2, MRP3, MRP4, MRP5 or MRP6 gene products.
Immunogén
Non-denatured membranes prepared from the human small cell lung cancer cell line, H69AR, which highly overexpresses MRP1 (Cole et al., 1992; Hipfner et al., 1994, 1999).
Alkalmazás
Anti-MRP1 Antibody, clone QCRL-1 detects level of MRP1 & has been published & validated for use in FC, WB, IC, IH, IH(P).
Western Blot: 0.2 μg/mL, with HRP-conjugated anti mouse IgG (Hipfner et al., 1994, 1996).
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Immunocytochemistry: 1:20 - 1:50 on paraformaldehyde-fixed (0.5% in PBS) or 70% methanol-fixed cytospin preparations (Hipfner et al., 1999).
Immunohistochemistry on formalin-fixed paraffini-embedded sections following antigen retrieval by microwave heating in citrate (Wright, et al., 1998).
Flow cytometry: 1:20 dilution on cells fixed in 0.5% paraformaldehyde in PBS for 30 minutes, followed by anti-mouse FITC (block and wash with solution containing 0.1% Tween) (Hipfner, et al., 1994).
Immunoprecipitation (Hipfner, et al., 1994; 1996).
Optimal working dilutions must be determined by the end user.
Fizikai forma
Format: Purified
Purified immunoglobulin. Liquid in PBS with 0.1% sodium azide.
Tárolás és stabilitás
Store at 2-8ºC for up to 3 months. For longer term storage, aliquot and freeze at -20ºC.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Nem találja a megfelelő terméket?
Próbálja ki a Termékválasztó eszköz. eszközt
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Cells resistant to HTI-286 do not overexpress P-glycoprotein but have reduced drug accumulation and a point mutation in alpha-tubulin.
Frank Loganzo, Malathi Hari, Tami Annable, Xingzhi Tan, Daniel B Morilla, Sylvia Musto et al.
Molecular Cancer Therapeutics null
M J Kelner et al.
Anti-cancer drugs, 11(3), 217-224 (2000-06-01)
This study is part of an effort to evaluate efficacy of the novel agent MGI 114 (HMAF) against tumors resistant to conventional chemotherapeutic agents. MGI 114 is a novel semisynthetic anticancer agent currently in chemotherapeutic phase II trials to evaluate
S P Cole et al.
Science (New York, N.Y.), 258(5088), 1650-1654 (1992-12-04)
The doxorubicin-selected lung cancer cell line H69AR is resistant to many chemotherapeutic agents. However, like most tumor samples from individuals with this disease, it does not overexpress P-glycoprotein, a transmembrane transport protein that is dependent on adenosine triphosphate (ATP) and
A rapid and quantitative coat protein complex II vesicle formation assay using luciferase reporters.
J Chris Fromme,Jinoh Kim
Analytical biochemistry null
B B Weksler et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(13), 1872-1874 (2005-09-06)
Establishment of a human model of the blood-brain barrier has proven to be a difficult goal. To accomplish this, normal human brain endothelial cells were transduced by lentiviral vectors incorporating human telomerase or SV40 T antigen. Among the many stable
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
Lépjen kapcsolatba a szaktanácsadással