AB1576
Anti-Cysteine String Protein Antibody
serum, Chemicon®
Szinonimák:
CSP, DnaJ Homologue Subfamily C member 5
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
serum
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás (homológia által előrejelzett)
all
gyártó/kereskedő neve
Chemicon®
technika/technikák
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... DNAJC5(80331)
Általános leírás
Cysteine-string proteins are membrane-bound proteins associated primarily with nerve endings and synaptic vesicles in the brain where they serve an important role in presynaptic function and may be involved in calcium-dependent neurotransmitter release at nerve endings. CSPs posses a strech of amino acids highly enriched in cysteine residues that are post-translationally palmitoylated. CSPs may function as homodimers.
Egyediség
Cysteine-string Protein. The antibody recognizes primarily the alpha isoform of CSP with some recognition of the beta isoforms. Slight reactivity with the gamma isoforms may be detected.
Immunogén
Recombinant rat cysteine-string protein with the cysteine string deleted.
Alkalmazás
Immunohistochemistry on formalin fixed tissue: 1:1,000. (Recommend Cat. No. AB1576P).Immunocytochemistry on PC-12 cells: 1:1,000. (Recommend Cat. No. AB1576P).Immunoblotting: 1:5,000. CSPs should be resolved on 12-15% SDS-PAGE gels under NON-REDUCING conditions. CSPs typically migrate as 33-35 kDa species in vertebrate samples (dimers may also be seen). If one must perform SDS-PAGE under reducing conditions, it is necessary to alkylate the CSPs to prevent smearing.To ensure proper identification of CSPs, samples may be deacylated by treatment with 1M neutral hydroxylamine or 0.1M KOH in methanol resulting in a 6-7 kDa shift in electrophoretic mobility of the CSP band. Deacylation will usually result imn some loss of reactivity on western blots so sufficient protein should be loaded. Alternatively, because all CSPs appear to be acidic (approximate pI of 4.6-4.8) 2-D gel electrophoresis may be usefull to distinguish some CSPs. Finally, some sample treatments may cause partial deacylation of CSPs resulting in a smear of immunoreactivity below the mass of the fatty acylated monomer.Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Cysteine String Protein Antibody is validated for use in WB, IC, IH for the detection of Cysteine String Protein.
Fizikai forma
Liquid rabbit serum containing no preservatives
Tárolás és stabilitás
Store at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Analízis megjegyzés
Control
POSITIVE CONTROL: brain samples
POSITIVE CONTROL: brain samples
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Huzefa Rupawala et al.
Brain communications, 4(4), fcac192-fcac192 (2022-08-06)
In Alzheimer's disease, synapse loss causes memory and cognitive impairment. However, the mechanisms underlying synaptic degeneration in Alzheimer's disease are not well understood. In the hippocampus, alterations in the level of cysteine string protein alpha, a molecular co-chaperone at the
Laura Caló et al.
Brain : a journal of neurology, 144(6), 1661-1669 (2021-03-25)
α-Synuclein aggregation at the synapse is an early event in Parkinson's disease and is associated with impaired striatal synaptic function and dopaminergic neuronal death. The cysteine string protein (CSPα) and α-synuclein have partially overlapping roles in maintaining synaptic function and
Evidence that the presynaptic vesicle protein CSPalpha is a key player in synaptic degeneration and protection in Alzheimer's disease.
Tiwari, SS; d'Orange, M; Troakes, C; Shurovi, BN; Engmann, O; Noble, W; Hortobagyi, T; Giese, KP
Molecular Brain null
Lukas Rüttiger et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(35), 12922-12927 (2004-08-26)
The large conductance voltage- and Ca2+-activated potassium (BK) channel has been suggested to play an important role in the signal transduction process of cochlear inner hair cells. BK channels have been shown to be composed of the pore-forming alpha-subunit coexpressed
Bruno A Benitez et al.
Acta neuropathologica communications, 3, 73-73 (2015-11-28)
Autosomal dominant adult-onset neuronal ceroid lipofuscinosis (AD-ANCL) is a multisystem disease caused by mutations in the DNAJC5 gene. DNAJC5 encodes Cysteine String Protein-alpha (CSPα), a putative synaptic protein. AD-ANCL has been traditionally considered a lysosomal storage disease based on the
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