AB1543P
Anti-Synapsin I Antibody
Chemicon®, from rabbit
Szinonimák:
Synapsin-1
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(4)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
klón:
polyclonal
application:
ELISA
ICC
IHC (p)
IP
WB
ICC
IHC (p)
IP
WB
faj reaktivitás:
human, mouse, rat, bovine
technika/technikák:
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
citations:
67
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
affinity purified immunoglobulin
antitest terméktípus
primary antibodies
klón
polyclonal
faj reaktivitás
human, mouse, rat, bovine
gyártó/kereskedő neve
Chemicon®
technika/technikák
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI elérési szám
UniProt elérési szám
kiszállítva
dry ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... SYN1(6853)
Általános leírás
The synapsins are a family of proteins that have long been implicated in the regulation of neurotransmitter release at synapses. Specifically, they are thought to be involved in regulating the number of synaptic vesicles available for release via exocytosis at any one time. Synapsins are encoded by three different genes, synapsin I, II and III, and different neuron terminals will encode different amounts of these; synapsin will make up 1% of total brain protein at any one time.
Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter.
Current studies suggest the following hypothesis for the role of synapsin: synapsins bind synaptic vesicles to components of the cytoskeleton which prevents them from migrating to the presynaptic membrane and releasing transmitter. During an action potential, synapsins are phosphorylated by Ca2+/calmodulin-dependent protein kinase II, releasing the synaptic vesicles and allowing them to move to the membrane and release their neurotransmitter.
Egyediség
Synapsin I (Mr of 80,000 and 77,000). Synapsin is located only in brain nerve terminal and is an excellent marker for synapes. Immunolabeling is blocked by preadsorption of the antibody with synapsin I.
Immunogén
Synapsin I (mixture of Ia & Ib) purified from bovine brain.
Alkalmazás
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Synapsin I Antibody is validated for use in ELISA, IC, IH, IH(P), IP, WB for the detection of Synapsin I.
Western blotting:
1:200-1:1,000 dilution of a previous lot was used.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein.
Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Immunohistochemistry:
1:500-1:2,500
ELISA:
1:2,500-1:10,00
Optimal working dilutions must be determined by the end user.
1:200-1:1,000 dilution of a previous lot was used.
Immunocytochemistry:
1:500-1:2,000 dilution of a previous lot was used.
Immunoprecipitation:
1 μg of a previous lot immunoprecipitated all of the synapsin I from a SDS homogenate of 200 μg of rat brain protein.
Note: The above dilutions are with 35S-protein A; with ECL dilutions may need to be considerably higher to obtain specific immunolabeling.
Immunohistochemistry:
1:500-1:2,500
ELISA:
1:2,500-1:10,00
Optimal working dilutions must be determined by the end user.
Minőség
Immunohistochemistry(paraffin):
Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval:
Rat Hippocampus
Synapsin I (AB1543P) representative staining pattern/morphology in rat hippocampal neurons. Tissue was pretreated with Citrate pH 6.0, antigen retrieval. This lot of antibody was diluted to 1:1000, IHC-Select reagents used with HRP-DAB. Immunoreactivity is seen directly associated with terminal end of neuronal cell body.
IHC-Paraffin Staining With Epitope Retrieval:
Rat Hippocampus
Cél megnevezése
77 & 80 kDa
Kapcsolódás
Replaces: MAB355
Fizikai forma
Format: Purified
ImmunoAffinity Purified
Purified rabbit polyclonal in buffer lyophilized from 5 mM ammonium bicarbonate. Reconstitute with 50 μL of PBS.
Tárolás és stabilitás
Stable for 1 year at -20ºC from date of receipt.
Analízis megjegyzés
Control
Brain tissue.
Brain tissue.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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javasolt
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 1
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Diffuse and specific tectopulvinar terminals in the tree shrew: synapses, synapsins, and synaptic potentials.
Wei, H; Masterson, SP; Petry, HM; Bickford, ME
Testing null
Valerie Kilman et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 22(4), 1328-1337 (2002-02-19)
Maintaining the proper balance between excitation and inhibition is necessary to prevent cortical circuits from either falling silent or generating epileptiform activity. One mechanism through which cortical networks maintain this balance is through the activity-dependent regulation of inhibition, but whether
Zi-Xuan He et al.
Cell reports, 33(5), 108343-108343 (2020-11-05)
Major depressive disorder (MDD) presents with two primary symptoms: depressed mood and anhedonia, which suggests that distinct neuronal circuits may regulate MDD. However, the underlying circuits of these individual symptoms linked to depression remain elusive. Herein, we identify a discrete
NMDA receptors mediate synaptic competition in culture.
She, K; Craig, AM
Testing null
Kyungmin Ji et al.
PloS one, 8(2), e56293-e56293 (2013-02-09)
Microglia are the immunocompetent cells of the central nervous system. In the physiological setting, their highly motile processes continually survey the local brain parenchyma and transiently contact synaptic elements. Although recent work has shown that the interaction of microglia with
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