MPEQ-AB is a 10X buffer that can serve as a wash buffer in the Cell Signaling kits when employing an automatic plate washer. It contains lower detergent concentrations compared to 43-041 and should not be utilized as the assay buffer in the kit. MPEQ-AB should specifically be used in the wash steps.
48-611MAG
MILLIPLEX® Akt/mTOR Phosphoprotein Magnetic Bead 11-Plex Kit - Cell Signaling Multiplex Assay
Szinonimák:
Akt/mTOR Signaling immunoassay, Luminex® Cell Signaling Assay, Millipore Cell Signaling Assay, Signal Transduction Assay
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Méret kiválasztása
96 EA
1 210 000,00 Ft
1 210 000,00 Ft
Product Availability: You will receive an order acknowledgement once the order has been entered in our system.
További információk kérése
Összes fotó(1)
Méret kiválasztása
Nézet módosítása
96 EA
1 210 000,00 Ft
About This Item
UNSPSC kód:
12161503
NACRES:
NA.47
1 210 000,00 Ft
Product Availability: You will receive an order acknowledgement once the order has been entered in our system.
További információk kérése
Javasolt termékek
faj reaktivitás
human
Minőségi szint
gyártó/kereskedő neve
Milliplex®
technika/technikák
multiplexing: suitable
észlelési módszer
fluorometric (Luminex® xMAP®)
tárolási hőmérséklet
2-8°C
Általános leírás
The Akt signaling pathway, one of the most often dysregulated signaling pathways in cancer, plays an important role in mediating a very broad range of cellular processes such as growth and development, cell cycle, energy homeostasis, and cell survival. Akt, a serine/threonine kinase that phosphorylates over 100 protein substrates, mediating cell survival and proliferation signals, is often itself hyperphosphorylated in various tumor types. Although the Akt gene itself is not known to be mutated in cancers, many of the upstream regulators of Akt, including IR, IRS, PI3K and PTEN, are oncogenes and tumor suppressors. Downstream of Akt, the mammalian Target Of Rapamycin (mTOR) complex is a key regulator of growth and metabolism. As nearly all of the players in the Akt/mTOR signaling pathway are coordinately regulated by phosphorylation, understanding the role of this pathway in normal physiological processes and in diseases such as cancer and diabetes requires the ability to simultaneously measure phosphorylation status of multiple protein targets.
Egyediség
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Alkalmazás
Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation and total pathway proteins in tissue and cell lysate samples. Compare Multiplexing results to those of Western blotting.
An overnight (4°C) incubation is recommended for best results.
This assay requires 25 μL diluted cell lysate per well.
This kit must be run using Assay Buffer 2 (provided).
The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
Analytes available:
An overnight (4°C) incubation is recommended for best results.
This assay requires 25 μL diluted cell lysate per well.
This kit must be run using Assay Buffer 2 (provided).
The suggested working range of protein concentration for the assay is 15 to 150 ng of total protein/well (25 μL/well at 600 to 6,000 ng/mL).
Analytes available:
- Akt (Ser473)
- GSK3α (Ser21)
- GSK3β (Ser9)
- IGF1R (Tyr1135/Tyr1136)
- IR (Tyr1162/Tyr1163)
- IRS1 (Ser636)
- mTOR (Ser2448)
- p70S6K (Thr412)
- PTEN (Ser380)
- RPS6 (Ser235/Ser236)
- TSC2 (Ser939)
Kiszerelés
96-well plate
Tárolás és stabilitás
Recommended storage for kit components is 2 - 8°C.
Jogi információk
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
Tárolási osztály kódja
10 - Combustible liquids
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Related Content
Learn how multiplexing with MILLIPLEX® multiplex assays can help simplify cancer research by measuring multiple circulating biomarkers at once. Find multiplex panels to analyze JAK-STAT, mTOR, MAPK, SAPK, TGFβ signaling pathways, and more.
Uncover how cells communicate with MILLIPLEX® cell signaling multiplex assays. Multiplexing with cell signaling phosphoprotein assays based on Luminex® xMAP® technology helps researchers measure phosphoproteins and total proteins within the same or different pathways from a single sample.
Fedezze fel a Luminex® xMAP® multiplex assay technológián alapuló MILLIPLEX® multiplex tesztek előnyeit, amelyek konzisztens, kiváló minőségű eredményeket biztosítanak, és nézze meg, hogyan használják ezeket a multiplex biomarker Luminex® teszteket a kutatás előmozdítására.
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What is the difference in formulation between the MPEQ-AB Assay Buffer and 43-041 Cell Signaling Assay Buffer 2? Can they be used interchangeably?
1 answer-
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