2502
ReBlot Plus Mild Antibody Stripping Solution, 10x
Szinonimák:
Western blot stripping solution, blot stripping solution
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41116010
eCl@ss:
42029053
technika/technikák:
western blot: suitable
application:
WB
citations:
11
Javasolt termékek
Forma
liquid
kiszerelés
pkg of 50 mL
gyártó/kereskedő neve
Chemicon®
Re-Blot™
technika/technikák
western blot: suitable
észlelési módszer
chemiluminescent
kiszállítva
wet ice
Általános leírás
Western blotting is a commonly used technique for studying protein function and localization. Typically, protein samples are electro-phoresed on SDS-PAGE and transferred to a membrane such as nitrocellulose or nylon, where they are probed with specific antibodies. Unlike nucleic acid based technologies, which allow reuse of Southern and Northern blots, it has been difficult to reuse Western blots.
Stripping and reprobing of Western blots offers several advantages:
1) Conservation of samples that are expensive or available only in limited quantities,
2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,
3) Reanalysis of anomalous results and confirmation with the same or a different antibody,
4) Correcting errors in incubation with the wrong antibody,
5) Cost savings in reagents and time by reusing the same blot.
While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.
The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.
Advantages of the Re-Blot Plus Western Blot Mild Antibody Stripping Solution include:
Stripping and reprobing of Western blots offers several advantages:
1) Conservation of samples that are expensive or available only in limited quantities,
2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,
3) Reanalysis of anomalous results and confirmation with the same or a different antibody,
4) Correcting errors in incubation with the wrong antibody,
5) Cost savings in reagents and time by reusing the same blot.
While antigen and antibody-based immunoaffinity matrices, such as Sepharose™ conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.
The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.
Advantages of the Re-Blot Plus Western Blot Mild Antibody Stripping Solution include:
- No pungent smelling β-mercaptoethanol is contained in the Antibody Stripping Solution.
- Antibody stripping is done at room temperature. No heating of blots is required.
- Blots can be stripped of antibodies in approximately 15 minutes at room temperature.
- Blots may be reused in 25 minutes.
Alkalmazás
The MILLIPORE Re-Blot Plus Western Blot Mild Antibody Stripping Solution is effective for removal of antibodies from Western blots that have been developed with chemiluminescence or radioactive iodine or other isotopes. It is not recommended for stripping colorimetric substrates (TMB, DAB, 4-chloronapthol, etc.), as it is not possible to effectively remove substrates that precipitate at the reaction site.
The Re-Blot Plus Western Blot Mild Antibody Stripping Solution should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.
This product is for research use only; not for diagnostic or in vivo use.
The Re-Blot Plus Western Blot Mild Antibody Stripping Solution should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.
This product is for research use only; not for diagnostic or in vivo use.
Komponensek
Mild Antibody Stripping Solution (10x) - (2 containers / 25 mL each).
Tárolás és stabilitás
The Mild Antibody Stripping Solution should be stored at 2-8°C upon arrival. Product is stable for 3 to 6 months after receipt. If Antibody Stripping Solution crystallizes upon storage, it may be re-dissolved with gentle warming at 37°C before use.
Note: To prevent reagent degradation secure the cap tightly upon storage. Avoid extended exposure to air.
Note: To prevent reagent degradation secure the cap tightly upon storage. Avoid extended exposure to air.
Jogi információk
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Re-Blot is a trademark of Merck KGaA, Darmstadt, Germany
Sepharose is a trademark of Cytiva
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A
Tárolási osztály kódja
6.1B - Non-combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 2
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Az ügyfelek ezeket is megtekintették
Cristian Mircean et al.
Bioinformatics (Oxford, England), 21(9), 1935-1942 (2005-01-14)
The protein lysate microarray is a developing proteomic technology for measuring protein expression levels in a large number of biological samples simultaneously. A challenge for accurate quantification is the relatively narrow dynamic range associated with the commonly used chromogenic signal
Nicholas W Baetz et al.
Molecular biology of the cell, 24(5), 643-658 (2013-01-04)
The Rab11-family interacting proteins (Rab11-FIPs) facilitate Rab11-dependent vesicle recycling. We hypothesized that Rab11-FIPs define discrete subdomains and carry out temporally distinct roles within the recycling system. We used live-cell deconvolution microscopy of HeLa cells expressing chimeric fluorescent Rab11-FIPs to examine
Jannike M Andersen et al.
Behavioural brain research, 390, 112676-112676 (2020-05-15)
Activation of calcium/calmodulin-dependent protein kinase II (CaMKII), particularly its α isoform, is known to be important for neuronal processes central for learning and memory and has also been implicated in the maladaptive learning involved in drug addiction.Thr286 autophosphorylation of αCaMKII
Xiaoyu Wang et al.
Proteome science, 9, 53-53 (2011-09-16)
An improved version of quantitative protein array platform utilizing linear Quantum dot signaling for systematically measuring protein levels and phosphorylation states is presented. The signals are amplified linearly by a confocal laser Quantum dot scanner resulting in ~1000-fold more sensitivity
C Baldock et al.
The Journal of cell biology, 152(5), 1045-1056 (2001-03-10)
We propose a new model for the alignment of fibrillin molecules within fibrillin microfibrils. Automated electron tomography was used to generate three-dimensional microfibril reconstructions to 18.6-A resolution, which revealed many new organizational details of untensioned microfibrils, including heart-shaped beads from
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