219364

Cathepsin B, Human Liver

Cathepsin B, Human Liver, CAS 9047-22-7, is a purified native cathepsin B from human liver, purified by affinity chromatography. Upregulated in many types of tumors.

• Szinonimák: Cathepsin B, Human Liver
• CAS-szám: 9047-22-7
• Enzyme Commission szám: 3.4.22.1 (BRENDA, IUBMB)
• UNSPSC kód: 12352202
• NACRES: NA.77

Tulajdonságok

• biológiai forrás: human liver
• Minőségi szint: 100
• Teszt: >95% (SDS-PAGE)
• form: liquid
• specifikus aktivitás: ≥50 units/mg protein
• tisztítva: affinity chromatography
• gyártó/kereskedő neve: Calbiochem^®
• tárolási körülmény: OK to freeze; avoid repeated freeze/thaw cycles
• technika/technikák: activity assay: suitable
• pH-tartomány: 3.5—6.0
• alkalmasság: suitable for molecular biology
• alkalmazás(ok): life science and biopharma
• kiszállítva: wet ice
• tárolási hőmérséklet: −70°C
• Géninformáció: human ... CTSB(1508)

Leírás

Általános leírás

Research area: Cell Signaling

Cathepsin B, Human Liver, is a native cathepsin B from human liver, purified by affinity chromatography. Cathepsin B is a lysosomal protease with endo/exopeptidase and a carboxydipeptidase activity. It is also known as cysteine cathepsin and belongs to the C1 family of papain-like enzymes. Cathepsin B like H, L, C, X, F, O and V is ubiquitously expressed in human tissues.

Optimal pH: 3.5-6.0.

Alkalmazás

Cathepsin B, Human Liver has been used to assess its linker cleavage or proteolytic activity using an LC/MS MRM-based quantitation method.

Biokémiai/fiziológiai hatások

Cathepsin B facilitates non-specific, bulk proteolysis within the acidic conditions of the endosomal/lysosomal compartment. Here, they break down both intracellular and extracellular proteins. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.

Kiszerelés

Please refer to vial label for lot-specific concentration.

Figyelmeztetés

Toxicity: Standard Handling (A)

Egység definíció

One unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-βNA per min at 40°C, pH 6.0 in the presence of DTT.

Fizikai forma

In 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.

Elkészítési megjegyzés

Prepared from tissue of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Feloldás

Following initial thaw, aliquot and freeze (-70°C).

Egyéb megjegyzések

Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol.389, 281.
Keppler, D., et al. 1994. Biochim. Biophys. Acta.1226, 117.
Musil, D., et al. 1991. EMBO J.10, 2321.
Sloane B.F. 1990. Semin. Cancer Biol.1, 137.

Jogi információk

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable