14-276-M
Akt1/PKBα Protein, active, 15 µg
Active, recombinant full-length human Akt1, containing N-terminal His6 tag, for use in IP-Kinase Assays & Kinase Assays.
Szinonimák:
Protein kinase B alpha, Proto-oncogene c-Akt, RAC, RAC-PK-alpha
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352202
eCl@ss:
32160405
NACRES:
NA.26
Javasolt termékek
biológiai forrás
human
Minőségi szint
rekombináns
expressed in baculovirus infected Sf21 cells
form
liquid
eltarthatósági idő
1 yr
molekulatömeg
Mw 59.9 kDa
gyártó/kereskedő neve
Upstate®
technika/technikák
activity assay: suitable (IP-kinase)
activity assay: suitable (kinase)
oldhatóság
soluble
NCBI elérési szám
UniProt elérési szám
tárolási hőmérséklet
−70°C
Géninformáció
human ... AKT1(207)
Általános leírás
Research area: CELL SIGNALING
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Product Source: Expressed by baculovirus in Sf21 insect cells
Recombinant full-length human Akt1, containing N-terminal His6-tag.
Biokémiai/fiziológiai hatások
Protein Target: Akt1/PKBα
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Target Sub-Family: AGC
Akt, also known as protein Kinase B (PKB), is a crucial protein involved in various cell signaling pathways. It modifies and controls the activities of numerous proteins involved in a wide range of cellular and physiological processes. Some of these processes include cell survival, apoptosis, cell proliferation, transcription, cell migration, and metabolism. Akt also helps in influencing angiogenesis, and normal development of embryos. Akt regulates cell survival through the phosphorylation of mitogen-activated protein kinase kinase kinase 5 (MAP3K5), which is involved in the cellular apoptotic response. The Akt occupies a pivotal position in the phosphoinositide 3-kinase-protein kinase B-mammalian target of rapamycin (PI3K-PKB-mTOR) signaling pathway. Genetic mutations occurring upstream of Akt in this cascade can result in uncontrolled cell proliferation and the development of cancer. In addition abberent Akt activation is associated with the development of type-2 diabetes.
Kiszerelés
Also available in 250μg size, please inquire for pricing and availability.
Minőség
Routinely evaluated by phosphorylation of Crosstide. Please refer to the Certificate of Analysis for more information.
Fizikai forma
Ni2+/NTA and glutathione agarose
Supplied in 50mM Tris/HCl pH7.5, 150mM NaCl, 150mM imidazole, 1mM benzamidine, 0.2mM PMSF, 0.1mM EGTA, 0.07% 2-mercaptoethanol, 0.02% Brij 35, 270mM sucrose. Frozen solution.
Tárolás és stabilitás
1 year at -70°C
Egyéb megjegyzések
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Jogi nyilatkozat
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2 - Skin Sens. 1
Tárolási osztály kódja
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 2
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Insulin Receptor Signaling
Laychock SG
Encyclopedia of Hormones, 368-380 (2003)
AKT/PKB signaling: navigating downstream
Manning BD, et al.
Cell, 129(7), 1261-1274 (2007)
Structural Biology and Anticancer Drug Design
Pathuri P, et al.
Journal of Separation Science, 2, 121-141 (2014)
The Impact of Mechanical Stress on the Pathophysiology of Osteoarthritis
Nuki G and Salter D
Journal of Separation Science, 33-52 (2007)
Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
Miinea, Cristinel P and Lienhard, Gustav E
Biotechniques, 34, 828-831 (2003)
Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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