07-448

Anti-monomethyl-Histone H3 (Lys27) Antibody

Upstate^®, from rabbit

• Szinonimák: H3K27me1, Histone H3 (mono methyl K27), H3 histone family, member T, histone 3, H3, histone cluster 3, H3, H3K27me1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• klón: polyclonal
• application: ChIP; DB; ICC; WB
• faj reaktivitás: human, vertebrates
• technika/technikák: ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 154

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: purified antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• faj reaktivitás: human, vertebrates
• gyártó/kereskedő neve: Upstate^®
• technika/technikák: ChIP: suitable (ChIP-seq); dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• izotípus: IgG
• NCBI elérési szám: NP_003484
• UniProt elérési szám: Q16695
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: monomethylation (Lys27)
• Géninformáció: human ... HIST1H3F(8968)

Leírás

Általános leírás

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

Egyediség

Broad species cross-reactivity expected, based on sequence homology.

Recognizes monomethylated histone H3 (Lys27), Mr 17 kDa.

Immunogén

Epitope: Lys27

KLH-conjugated, synthetic 2X-branched peptide containing the sequence …ARmeKSA… in which meK corresponds to monomethyl-lysine at residue 27 of human histone H3.

Alkalmazás

Anti-monomethyl-Histone H3 (Lys27) Antibody is a rabbit polyclonal antibody to detect Histone H3 monomethylated at lysine 27. This purified Ab, also known as Anti-H3K27me1 is specificity verified by dot blot (DB), published in peer reviewed journals and validated in ICC, WB, ChIP, ChIP-seq.

Immunocytochemistry & Dot Blot:
Reported by an independent laboratory (Kohlmaier, A., 2004; Perez-Burgos, L., 2004; Peters, A. H., 2003).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Minőség

Routinely evaluated by western blot acid in extracted proteins from Hela cells.

Western Blot Analysis:
0.1-0.2 μg/mL had detected monomethylated histone H3 in acid extracted proteins from HeLa cells.

Cél megnevezése

17 kDa

Fizikai forma

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
HeLa acid extract

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi információk

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable