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Merck
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Fontos dokumentumok

07-2268

Sigma-Aldrich

Anti-phospho-TBC1D1 Antibody (Ser237)

from rabbit, purified by affinity chromatography

Szinonimák:

TBC1 domain family member 1, TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
12352203
eCl@ss:
32160702

biológiai forrás

rabbit

Minőségi szint

100

antitest terméktípus

primary antibodies

klón

polyclonal

tisztítva

affinity chromatography

faj reaktivitás

human

faj reaktivitás (homológia által előrejelzett)

ox (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), mouse (based on 100% sequence homology), rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

technika/technikák

western blot: suitable

NCBI elérési szám

NP_055988

UniProt elérési szám

Q86TI0

kiszállítva

wet ice

célzott transzláció utáni módosítás

phosphorylation (pSer237)

Géninformáció

human ... TBC1D1(23216)

Általános leírás

TBC1D1 is the first member of a family of proteins that contain the tbc box motif of 180-220 amino acids and may be related to cell growth and differentiation. The composition of TBC1D1 has the same function as a known regulator of insulin-mediated Glut4 translocation and may play a role in severe human obesity. An insulin-independent increase in glucose transport several hours after exercise has been attributed to TBC1D1 phosphorylation. The R125W mutation of TBC1D1 is thought to decrease skeletal muscle glucose transport and could be the main reason for the obesity that seems to be linked to this type of mutation.

Egyediség

This antibody recognizes TBC1D1 phosphorylated at Ser237.

Immunogen

Epitope: Phosphorylated Ser237
KLH-conjugated linear peptide corresponding to human TBC1D1 phosphorylated at Ser237.

Alkalmazás

Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling

Glucose/Glycogen Metabolism
This phospho-TBC1D1 antibody is validated for use in WB for the detection of the phospho-TBC1D1 protein.

Minőség

Evaluated by Western Blot in lambda phosphatase treated and untreated HEK293 cell lysates.

Western Blot Analysis: 1 µg/mL of this antibody detected TBC1D1 on 10 µg of lambda phosphatase treated and untreated HEK293 cell lysates.

Cél megnevezése

~145 kDa observed

Fizikai forma

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Analízis megjegyzés

Control
Lambda phosphatase treated and untreated HEK293 cell lysates

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

12 - Non Combustible Liquids

WGK

WGK 1

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Marie Locard-Paulet et al.
Molecular systems biology, 16(7), e9524-e9524 (2020-07-04)
T-cell receptor (TCR) ligation-mediated protein phosphorylation regulates the activation, cellular responses, and fates of T cells. Here, we used time-resolved high-resolution phosphoproteomics to identify, quantify, and characterize the phosphorylation dynamics of thousands of phosphorylation sites in primary T cells during
Sharon C Hook et al.
Scientific reports, 10(1), 17953-17953 (2020-10-23)
Proteins involved in the spaciotemporal regulation of GLUT4 trafficking represent potential therapeutic targets for the treatment of insulin resistance and type 2 diabetes. A key regulator of insulin- and exercise-stimulated glucose uptake and GLUT4 trafficking is TBC1D1. This study aimed
Jonas Roland Knudsen et al.
Experimental physiology, 104(5), 704-714 (2019-02-03)
What is the central question of this study? Resolving the mechanism(s) leading to glucose transporter 4 (GLUT4) translocation to the muscle surface membrane has great therapeutic potential. However, the measurement of GLUT4 translocation is technically challenging. Here, we asked whether
Yang Liu et al.
Nature, 578(7796), 605-609 (2020-02-14)
The activation of adenosine monophosphate-activated protein kinase (AMPK) in skeletal muscle coordinates systemic metabolic responses to exercise1. Autophagy-a lysosomal degradation pathway that maintains cellular homeostasis2-is upregulated during exercise, and a core autophagy protein, beclin 1, is required for AMPK activation in

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