06-680

Anti-Androgen Receptor Antibody

from rabbit, purified by affinity chromatography

• Szinonimák: Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 100
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• tisztítva: affinity chromatography
• faj reaktivitás: rat, human
• faj reaktivitás (homológia által előrejelzett): bovine (based on 100% sequence homology), canine (based on 100% sequence homology), horse (based on 100% sequence homology), mouse (based on 100% sequence homology)
• technika/technikák: ChIP: suitable (ChIP-seq); flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI elérési szám: NP_036634
• UniProt elérési szám: P15207
• kiszállítva: wet ice
• Géninformáció: bovine ... Ar(280675) ; dog ... Ar(403588) ; human ... AR(367) ; mouse ... Ar(11835) ; rat ... Ar(24208)

Leírás

Általános leírás

The androgen receptor (AR) is a type of nuclear receptor which is activated by binding of either of the androgenic hormones testosterone or dihydrotestosterone. The main function of the androgen receptor is as a DNA binding transcription factor which regulates gene expression. However the androgen receptor also has additional functions independent of DNA binding. The androgen receptor is most closely related to the progesterone receptor, and progestins in higher dosages can block the androgen receptor.

Immunogen

Epitope: This antibody recognizes the Modulation Region within the N-terminus of Androgen Receptor.

KLH-conjugated linear peptide corresponding to the N-terminus of rat Androgen Receptor.

Alkalmazás

Anti-Androgen Receptor Antibody, PG-21 is a Rabbit Polyclonal Antibody for detection of Androgen Receptor also known as Dihydrotestosterone Receptor & has been tested in ICC, IHC, IP & WB.

Immunoprecipitation Analysis: 34 µL from a representative lot immunoprecipitated Androgen Receptor from 1000 µg of LNCaP cell lysate.

Flow Cytometry Analysis: A 1:300 dilution from a representative lot detected Androgen Receptor in 0.2X10E6 MCF-7 cells.

Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from DU145 cells stably transfected with a 7 kD-PSA promoter, different AR poly A lentiviral vectors, and a Flag-tagged hZMIZ1 vector (Li, X., et al. (2011). PLoS One. 6(9):e25040.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in embryonic rat tissue, neonatal rat testis tissue, and adult rat testis tissue (Nation, T., et al. (2010). J Pediatr Surg. 46(8): 1539-1543.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected Androgen Receptor in MCF-7 cells stabily transfected with Androgen Receptor (Ghatge, R. P., et al. (2005). Breast Cancer Res. 7(6):R1036-1050.).

Chromatin Immunoprecipitaton-Sequence Analysis: A representative lot from an independent laboratory immunoprecipitated Androgen Receptor from LNCaP cell lysates under certain treated conditions (Zhao, J. C., et al. (2012). Genome Res. 22(2):322-331.).

Immunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Androgen Receptor in LNCaP cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Minőség

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Androgen Receptor in 10 µg of LNCaP cell lysate.

Cél megnevezése

~110 kDa observed

Fizikai forma

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable