05-928
Anti-Histone H3 Antibody, CT, pan, clone A3S, rabbit monoclonal
clone A3S, Upstate®, from rabbit
Szinonimák:
H3, Histone H3, H3 histone, family 3A
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
UNSPSC kód:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Javasolt termékek
biológiai forrás
rabbit
Minőségi szint
antitest forma
purified antibody
antitest terméktípus
primary antibodies
klón
A3S, monoclonal
faj reaktivitás
Saccharomyces cerevisiae, mouse, chicken, yeast, human, rat
gyártó/kereskedő neve
Upstate®
technika/technikák
ChIP: suitable
western blot: suitable
izotípus
IgG
NCBI elérési szám
UniProt elérési szám
kiszállítva
wet ice
célzott transzláció utáni módosítás
unmodified
Géninformáció
human ... H3F3B(3021)
Általános leírás
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
Egyediség
Broad species cross-reactivity expected due to sequence homology.
Recognizes Histone H3, Mr 17 kDa
Immunogen
KLH-conjugated, synthetic peptide corresponding to the C-terminus of human Histone H3.
Alkalmazás
Chromatin Immunoprecipitation:
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
2 μL of this antibody immunoprecipitated chromatin associated with Histone H3 from a wild type yeast lysate.
Use Anti-Histone H3 Antibody, CT, pan, clone A3S (Rabbit Monoclonal Antibody) validated in ChIP, WB to detect Histone H3 also known as Histone H3.
Minőség
Routinely evaluated by western blot analysis.
Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Western Blot Analysis:
1:2,000-1:20,000 dilution of this lot detected Histone H3 in a modification independent manner in acid extracted proteins from untreated, sodium butyrate or colcemid treated HeLa cells.
Cél megnevezése
17 kDa
Fizikai forma
Format: Purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol.
Tárolás és stabilitás
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analízis megjegyzés
Control
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.
Acid extracted proteins from HeLa cells, HeLa nuclear extracts or acid extracted HeLa cells treated with sodium butyrate.
Egyéb megjegyzések
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Jogi információk
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 2
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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