05-1341

Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301

clone CMA301, from mouse, purified by affinity chromatography

• Szinonimák: H3 histone family, member T, histone 3, H3, histone cluster 3, H3
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: affinity purified immunoglobulin
• klón: CMA301, monoclonal
• tisztítva: affinity chromatography
• faj reaktivitás: vertebrates, human
• technika/technikák: ELISA: suitable; dot blot: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• izotípus: IgG2bκ
• NCBI elérési szám: NM_003493
• UniProt elérési szám: Q16695
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. The term "Histone H3" alone is purposely ambiguous in that it does not distinguish between sequence variants or modification state. Histone H3 is an important protein in the emerging field of epigenetics, where its sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes.

Egyediség

Broad species cross-reactivity is expected, based on sequence homology.

This antibody recognizes Histone H3 that is unmodified at Thr3 and Lys4 but does not recognize phosphorylated Thr3 nor acetylated or methylated Lys4. The antibody binding specificity allows modifications of Lys9, Ser10, and/or Lys141.

Immunogen

Epitope: N-terminus (unmodified)

Synthetic peptide corresponding to amino acids 1-12 of human Histone H3, conjugated to KLH.

Alkalmazás

ELISA:
This antibody has been shown by an outside laboratory to be suitable for ELISA.1

Immunocytochemistry:
This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.1

Immunoprecipitation:
This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.1

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Use Anti-Histone H3 Antibody, (Unmodified Lys4), clone CMA301, (Mouse Monoclonal Antibody) validated in WB, ICC, ELISA, IP, FC, DB to detect Histone H3 also known as histone 3-H3.

Minőség

Routinely evaluated by Western Blot on HeLa cell lysate.

Western Blot Analysis: 1:10,000 dilution of this antibody detected histone H3 in 10 μg of HeLa Acid Extract cell lysate and recombinant histone H3 (500 ng) but not other recombinant histones.

Cél megnevezése

Approx. 17 kDa

Fizikai forma

Mouse monoclonal IgG2bκ supernatant in PBS with 0.05% sodium azide.

Peptide affinity chromatography

Tárolás és stabilitás

Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analízis megjegyzés

Control
HeLa Acid extract lysate.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable