05-1140

Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18

clone 18, from mouse

• Szinonimák: FADK 1, PTK2 protein tyrosine kinase 2, Protein-tyrosine kinase 2, focal adhesion kinase 1
• UNSPSC kód: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 100
• antitest forma: purified immunoglobulin
• antitest terméktípus: primary antibodies
• klón: 18, monoclonal
• faj reaktivitás: mouse, human
• technika/technikák: western blot: suitable
• izotípus: IgG1
• UniProt elérési szám: Q05397
• kiszállítva: wet ice
• célzott transzláció utáni módosítás: phosphorylation (pTyr397)
• Géninformáció: human ... PTK2(5747) ; mouse ... Ptk2(14083)

Leírás

Általános leírás

FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle. FAK regulation includes phosphorylation at multiple tyrosine and serine residues. Phosphorylation of tyrosine generally is associated with positive regulation and growth promotion, however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase of the cell cycle). In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly. Tyrosine 397 is the autophosphorylation site of Focal Adhesion Kinase. The site binds Src family SH2 domains and the p85 subunit of PI3-Kinase.

Egyediség

Reacts specifically with Focal Adhesion Kinase (FAK) when phosphorylated on Tyr397. FAK is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the C-terminus called the "Focal Adhesion Targeting" sequence (FAT). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at Tyr-397, and activation of FAK through phosphorylation of Tyr residues (Tyr-576 and Tyr577) in the kinase domain activation loop.

Immunogén

Epitope: Tyrosine 397

Generated from human FAK, a.a. 393-404, phosphorylated on Tyr397.

Alkalmazás

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-phospho-Focal Adhesion Kinase (Tyr397) Antibody, clone 18 is validated for use in WB for the detection of phospho-Focal Adhesion Kinase (Tyr397).

Minőség

Evaluated by Western Blot in LPS treated RAW 264 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-FAK (Tyr397) on 10 ug of LPS treated RAW 264 lysates.

Cél megnevezése

125 kDa

Kapcsolódás

Replaces: 04-974

Fizikai forma

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA, and <0.09% sodium azide.

Tárolás és stabilitás

Stable for 1 year at -20ºC from date of receipt.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analízis megjegyzés

Control
LPS treated RAW 264 lysates.

Egyéb megjegyzések

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable