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Merck

A4503

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

cold ethanol fraction, pH 5.2, ≥96%

别名:

BSA, 牛白蛋白, 牛血清白蛋白

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

方案

≥96%

表单

lyophilized powder

分子量

~66 kDa

纯化方式

cold ethanol fractionation

来源

USA origin

技术

ELISA: suitable
blood typing: suitable
microbiological culture: suitable

杂质

BT and VSV virus, tested

缺失

≤5%

pH值(酸碱度)

5.2

溶解性

water: soluble (40 mg/ml)

UniProt登记号

异质活性

BT Virus, none detected
VSV Virus, none detected

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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一般描述

Bovine serum albumin is a model protein in various studies and is made up of 583 amino acids. It is a water-soluble protein with a molecular weight of 66.4kDa. Six α-helices form three homologous domains of BSA. Depending on pH, it undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.

应用

  • Bovine Serum Albumin has been used in solution prepared for the isolation of heart mitochondria.
  • It has been used in the cytotoxicity assay buffer.
  • It has been used for the induction of protein overload nephropathy in rats.

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

制备说明

血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此,有些人便开始使用“Fraction V”这一术语来描述血清白蛋白,无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白,而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白,包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。
通常称为 Cohn fraction V;本品采用改进的 Cohn 氏冷乙醇分级分离法制备。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Sensitization of tumor cells to NK cell-mediated killing by proteasome inhibition.
Hallett WH et al.
Journal of Immunology, 180, 163-163 (2008)
Cytochrome C effect on respiration of heart mitochondria: influence of various factors.
Toleikis A et al.
Bioscience Reports, 25, 387-387 (2005)
Isabella Marcomini et al.
Cell reports, 24(10), 2614-2628 (2018-09-06)
Multiple pathways regulate the repair of double-strand breaks (DSBs) to suppress potentially dangerous ectopic recombination. Both sequence and chromatin context are thought to influence pathway choice between non-homologous end-joining (NHEJ) and homology-driven recombination. To test the effect of repetitive sequences
Rapamycin worsens renal function and intratubular cast formation in protein overload nephropathy.
Coombes JD et al.
Kidney International, 68, 2599-2599 (2005)
Simona Ranallo et al.
Nature communications, 8, 15150-15150 (2017-05-10)
A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we

商品

本页将全面阐述血清白蛋白和其他重要细胞培养成分如何影响用于单克隆抗体等外源蛋白生物制造的无血清细胞培养系统的性能,并介绍白蛋白的体外化学和生物化学过程。本页面将介绍白蛋白在体外化学和生物化学领域的应用。这些深度知识可能也适用于离体和组织工程学应用。

Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.

实验方案

Spectrophotometric assay at 260 nm measures nuclease S1 activity, vital for nucleic acid research, with defined enzyme unit criteria.

This procedure applies to all products from heart muscle that have a specification for L-Lactic Dehydrogenase activity.

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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相关内容

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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