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XTGHP-RO

Roche

X-tremeGENE™ HP DNA转染试剂

High-performance polymer reagent for transfecting many cell lines

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About This Item

分類程式碼代碼:
41106502
NACRES:
NA.55

等級

for molecular biology

品質等級

100

形狀

liquid (aqueous solution)

用途

 mL (suitable for 165 transfections)

包裝

pkg of 0.4 mL (06366244001)
pkg of 1.0 mL (06366236001)
pkg of 5 × 1 mL (06366546001)

製造商／商標名

Roche

技術

transfection: suitable

儲存溫度

−20°C

相关类别

一般說明

X-tremeGENE HP DNA转染试剂是一款多组分试剂，能够与DNA形成复合物，并将其递送至动物或昆虫细胞中。

特點和優勢

X-tremeGENE HP DNA转染试剂是一种高效转染试剂，可成功转染多种真核细胞，包括昆虫细胞和难转染细胞系（如Sf9、U-2 OS、MEF、MCF7、Hep G2、PC-3、HeLa、HT-29、HT-1080、K-562、RAW 264.7、Jurkat、PC-12和HCT 116）。

  • 简单易用的非脂质体试剂 无动物源性成分，室温稳定，经0.2 μm滤膜过滤，并且在有血清培养液中仍保持活性。
  • 高转染效率 对于原代细胞和使用其它试剂难转染的肿瘤细胞系有高转染效率。
  • 结果生理相关性好 使用细胞毒性低的试剂。
  • 满足高通量实验 实现简单一致的靶标评估。

品質

每一批次的X-tremeGENE HP DNA转染试剂都经过严格的质量控制流程测试，确保性能合规的标准性。在质量测试环节，首先使用X-tremeGENE HP DNA转染试剂（试剂DNA比为3:1μl/μg DNA）向细胞转染一个报告基因载体。再通过化学荧光检测法监测报告基因活性。最后使用一条标准曲线对每孔的重组蛋白总量进行定量，以确定产品达到规格。

外觀

本产品溶于80%乙醇中，经0.2 μm滤膜过滤。无人源或动物源成分。次数：使用标准步骤时，在96孔板内，1ml X-tremeGENE™ HP DNA转染试剂可以进行10,000次转染。

其他說明

仅用于生命科学研究。不可用于诊断。

法律資訊

X-tremeGENE is a trademark of Roche

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象形圖

FlameExclamation mark
GHS02,GHS07

訊號詞

Danger

危險聲明

H225 - H319

危險分類

Eye Irrit. 2 - Flam. Liq. 2

儲存類別代碼

3 - Flammable liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

334.4 °F

閃點（°C）

168 °C

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Abazar Roustazadeh et al.
SpringerPlus, 5(1), 711-711 (2016-07-05)
Matrix Gla protein (MGP) is involved in calcium trafficking and arterial calcification. The aim of study was to investigate the role of three polymorphisms within the MGP gene promoter region on reporter gene (luciferase) expression level. The fragments containing rs1800799
Takashi Yoshino et al.
Cellular and molecular neurobiology, 31(7), 1009-1020 (2011-04-27)
A synthetic analog of sphingosine named FTY720 (Fingolimod), phosphorylated by sphingosine kinase-2, interacts with sphingosine-1-phosphate (S1P) receptors expressed on various cells. FTY720 suppresses the disease activity of multiple sclerosis (MS) chiefly by inhibiting S1P-dependent egress of autoreactive T lymphocytes from
Laura M Griffin et al.
Methods in molecular biology (Clifton, N.J.), 1195, 219-238 (2013-11-28)
Human papillomaviruses (HPVs) are non-enveloped DNA viruses that are highly tropic for mucosal and cutaneous epithelia. The HPV life cycle is tightly linked to epithelial cell differentiation, where HPVs only infect the basal proliferating keratinocytes, and progeny virus assembly and
Jihoon Kim et al.
Bioorganic & medicinal chemistry, 19(24), 7582-7589 (2011-11-11)
The signal transducer and activator of transcription 3 (STAT3) is constitutively activated in cancer cells. Therefore, blocking the aberrant activity of STAT3 in tumor cells is a validated therapeutic strategy. To discover novel inhibitors of STAT3 activity, we screened against
Isabelle Coupienne et al.
Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 10(12), 1868-1878 (2011-10-29)
Glioblastoma constitute the most frequent and deadliest brain tumors of astrocytic origin. They are resistant to all current therapies and are associated with a high rate of recurrence. Glioblastoma were previously shown to respond to treatments by 5-aminolevulinic acid (5-ALA)-based
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实验方案

使用X-tremeGENE HP转染试剂进行慢病毒生产

慢病毒是一种用于在研究应用中转染多种细胞类型的强大工具。

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X-tremeGENE™ HP DNA转染试剂实验方案

约转染前24小时接种转染细胞，确保细胞处于最佳密度（7090 %融合度）。

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

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我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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