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HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide

HPLC Analysis of Water Soluble B Vitamins on Ascentis® Express RP-Amide application for HPLC

Materiály

Application

Č. produktu
Popis
Stanovení ceny

Ascentis® Express RP-Amide, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 5 cm × 3 mm

Standard

Č. produktu
Popis
Stanovení ceny

Pyridoxine hydrochloride

≥98% (HPLC)

Calcium pantothenate

meets USP testing specifications, monograph mol wt. 476.53 (C18H32CaN2O10)

Thiamine hydrochloride

reagent grade, ≥99% (HPLC)

Nicotinic acid

≥99.5% (HPLC)

Nicotinamide

≥98.5% (HPLC)

Biotin

≥99.0% (T)

Cyanocobalamin (B12)

analytical standard

Riboflavin (B2)

analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3.0 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] 20 mM potassium phosphate, dibasic, pH 6.2; [B] acetonitrile

gradient

0% B for 0.5 min, to 15% B over 0.3 min; up to 35% B in 1.2 min, 1 min equilibration at 0% B

flow rate

2.0 mL/min

pressure

5076 psi (350 bar)

column temp.

35 °C

detector

UV, 210 nm

injection

5 μL

sample

9 vitamins in water, 10 μg/mL for nicotinic acid and folic acid, 12 μg/mL for thiamine hydrochloride and cyanocobalamin, (20 μg/mL for pyridoxine, 30 μg/mL for riboflavin, 40 μg/mL for niacineamide, 100 μg/mL for calcium pantothenate, 120 μg/mL for biotin)

Popis

Analysis Note

A mix of 9 water-soluble B-vitamins was separated on Ascentis Express RPA column

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany