HPLC Analysis of Serotonin Metabolites on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 10 mM formic acid/acetate (pH 3.0): [B] methanol; (80:20, A:B)

flow rate

0.7 mL/min

column temp.

35 °C

detector

ESI (+); overlay of extracted ion chromatograms of individual (M+H)+ species

injection

10 μL

sample

25 mg/L in 90:10, 10 mM formic acid/acetate (pH 3.0):methanol