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HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl

HPLC Analysis of Fusarium Mycotoxins on Ascentis® Express Phenyl-Hexyl application for HPLC

Materiály

analytical column

Č. produktu
Popis
Stanovení ceny

Ascentis® Express Phenyl-Hexyl, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Č. produktu
Popis
Stanovení ceny

Nivalenol solution

100 μg/mL in acetonitrile, analytical standard

15-Acetyldeoxynivalenol

reference material

3-Acetyldeoxynivalenol

CONDITIONS

column

Ascentis Express Phenyl-Hexyl, 10 cm x 2.1 mm I.D., 2.7 μm particles (53336-U)

mobile phase

[A] water, acetonitrile, 90:10; [B] acetonitrile

gradient

0% B held for 0.5 min; to 15% B in 1 min; held at 15% B for 6 min

flow rate

0.3 mL/min

pressure

192 bar (2780 psi)

column temp.

30 °C

detector

UV, 218 nm

injection

10 μL

sample

5 mg/L ea. in 95:5, water:acetonitrile

Popis

Analysis Note

The trichothecene vomitoxins of Fusarium are significant plant pathogens of grain and forage crops that can lead to anorexic effects with livestock. This application demonstrates resolution of deoxynivalenol and three of its analogs.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany