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HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5 application for HPLC

Materiály

Analytical column

Č. produktu
Popis
Stanovení ceny

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 7.5 cm × 3 mm

Standard

Č. produktu
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Stanovení ceny

Codeine monohydrate

analytical standard

Morphine sulfate salt pentahydrate

analytical standard

Benzoylecgonine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Fentanyl solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Hydrocodone solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(±)-MDMA solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(±)-Methamphetamine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Meperidine solution

1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

Normeperidine solution

100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

(+)-Propoxyphene solution

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

CONDITIONS

column

Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)

mobile phase

[A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water

gradient

held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min

flow rate

0.3 mL/min

pressure

175 bar

column temp.

35 °C

detector

ESI(+), m/z 100-1000

injection

1 μL

sample

1 μg/mL in 50:50, water: methanol

Popis

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany