HPLC Analysis of Benzalkonium Chloride in Commercial Disinfecting Wipes on Ascentis^® Express HILIC

column

Ascentis Express HILIC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] 20 mM ammonium acetate; [B] acetonitrile; (10:90, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

ESI(+), TIC 150 - 500 m/z

injection

2 μL

sample

Single wipe sheet, extracted with 10 mL methanol