HPLC Analysis of Amphetine, Methamphetamine, MDA, and MDMA on Discovery^® HS F5

column

Discovery HS F5, 15 cm × 4.6 mm I.D., 5 μm particles (567516-U)

mobile phase

[A] 10 mM ammonium acetate in [B] water: acetonitrile (10:90)

flow rate

1.5 mL/min

column temp.

35 °C

detector

UV, 210 nm

injection

5 μL

sample

50 μg/mL each (amphetamine, methamphetamine, MDA and MDMA) in methanol