SSN-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96082808, fish unknown/unspecified, Fibroblast

• UNSPSC Code: 41106514

Vlastnosti

• biological source: fish unknown/unspecified
• packaging: tube of 5 μg 96082808-DNA-5UG; pkg of vial of cells 96082808-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Fish striped snakehead fry, spontaneous production of c-type retrovirus

Cell Line Description

The fish cell line SSN-1 was initiated from whole fry tissue, Channa (Ophicephalus) striatus, commonly named ′striped snakehead′. SSN-1 spontaneously produce and release endogenous snakehead fish Mn²+- dependent C-type retrovirus. The cell line is suitable for fish virus isolation and is susceptible to epizootic ulcerative syndrome rhabdoviruses and piscine nodaviruses. SSN-1 was previously infected with mycoplasma but has been eradicated and tested negative after a minimum of 10 passages.

Application

Isolation of fish virus pathogens

Culture Medium

L15 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. Use trypsin very cautiously; 25-30°C, can be light sensitive therefore avoid prolonged exposure to bright light. Fish cell lines detach easily during transit if the culture is very young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

When resuscitating a frozen ampoule of cells we recommend the cells are seeded at the higher seeding level i.e. 4 x10, 000/cm². Cells may take up to 10 days to reach 70% following resuscitation and subculture. Therefore media change flasks after 4-5 days to encourage growth.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.