SHK-1

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 97111106, fish unknown/unspecified, Fibroblast-like

• UNSPSC Code: 41106514
• Biological source: fish unknown/unspecified
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast-like
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: fish unknown/unspecified
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 97111106-DNA-5UG, pkg of vial of cells 97111106-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Propagation of infectious salmon anaemia (ISA) virus

SHK-1 cell line has been used to determine the effects of different nucleotide concentrations on cell viability. It has also been used to study the effects of Silymarin, a compound used in aquaculture.

Biochem/physiol Actions

Atlantic salmon head kidney

The cell line was established from primary cultures of adherent cells from Atlantic salmon head kidney. The cells have been used in the study of the infectious salmon anaemia (ISA) virus. SHK-1 cells are recognised by MAbs specific for Atlantic salmon peripheral blood leucocytes, but not with Mabs specific with polymorphonuclear leucocytes. SHK-1 cells have been shown to phagocytose the fish pathogen Aeromonas salmonicidia to some extent, but no bacterical activity was observed over a period of 72 hours. From these results it has been concluded that SHK-1 is derived from leucocytes with some of the properties of macrophages. Studies have shown that the cells can be passaged more than 60 times.

Preparation Note

Leibovitz L15 + 4mM Glutamine + 40μM 2-Me + 5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 seeding at 5 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA. Incubate at 20°C. CO₂ must not be used. Keep culture dark e.g. by wrapping flask in foil. The cells are sensitive to trypsin which must be removed at each subculture by centrifuging the harvested cells at 100 x g for 5 minutes and resuspending the cell pellet in fresh medium. Cells grow better if conditioned medium is used at each stage supplemented with fresh medium.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Birgit H Dannevig, National Veterinary Institute, Ullevaalsveien 68; Oslo

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.