BF-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21712, fish caudal trunk, Fibroblast-like

• UNSPSC Code: 41106514
• Biological source: fish caudal trunk
• Growth mode: Adherent
• Karyotype: 2n=48, stem-line = 48 with scattering from 36-121
• Morphology: Fibroblast-like
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: fish caudal trunk
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 00021712-DNA-5UG, pkg of vial of cells 00021712-1VL
• growth mode: Adherent
• karyotype: 2n=48, stem-line = 48 with scattering from 36-121
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Biochem/physiol Actions

Fish, bluegill fry

The cell line BF-2 has been derived from a trypsinised suspension of pooled caudal portions of the trunk of 1 year old fingerlings of Bluegill fry (Lepomis macrochirus). Cells have been reported to be susceptible to a variety of fish viruses including lymphocystis virus, viral haemorrhagic septicaemia virus (VHSV), infectious pancreatic necrosis virus (IPNV) and tadpole edema virus (TEV) of frogs. The cell monolayer is confluent after 48-56 hours using a 1:4 split ratio. Catalogue no. at CCLV (depositor) is RIE 193.

Preparation Note

EMEM(EBSS) (M2279) + 2mM L-Glutamine (G7513) + 1% Non Essential Amino Acids(NEAA) (M7145) + 120 mg/l Sodium Pyruvate(NaP) (S8636) + 10% FBS / FCS (F2442).

Split sub-confluent cultures (70-80%) 1:4 to 1:6 using 0.25% trypsin or trypsin/EDTA; cells replicate at 15-33ºC, optimal temperature 23-26ºC; monolayer is stable for at least 10 days, maintenance medium should contain MEM(EBSS) + 740 mg/l NaHCO₃ + 1.2g/l

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.