NB4 1A3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: mouse nerve
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Unstable
• Morphology: Neuroblast
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: mouse nerve
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89121405-DNA-5UG, pkg of vial of cells 89121405-1VL
• growth mode: Adherent
• karyotype: Unstable
• morphology: Neuroblast
• products: Acetylcholinesterase, choline acetylase, tyrosine hydroxylase
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Biochem/physiol Actions

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.

Mouse C-1300 Neuroblastoma

Preparation Note

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.