Grunt Fin (GF)

88010601, fish fin, Fibroblast-like

• UNSPSC Code: 41106514

Vlastnosti

• product name: Grunt Fin (GF), 88010601
• biological source: fish fin
• growth mode: Adherent
• karyotype: Modal no. 47
• morphology: Fibroblast-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Fish Grunt fin tissue blue striped

Cell Line Description

Derived from fin tissue of the adult salt water blue striped Grunt. Does not support the replication of influenza A, herpes simplex, adeno, polio, dengue and eastern equine encephalitis viruses.

Culture Medium

EMEM (HBSS) (Sigma cat no. M5775) + 10mM Hepes (pH 7.3) + 2mM Glutamine + 0.23% (w/v) NaHCO₃ + 20% FBS (Heat Inactivated)+ 58mM NaCl

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 20°C. After resuscitation split cells 1:2 to 1:4 and culture for at least one week with 1-2 media changes before shipping. Plating efficiency can be very low a

Other Notes

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