CAR

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: fish fin
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Fibroblast
• Manufacturer/tradename: ECACC

Vlastnosti

• biological source: fish fin
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 89072611-DNA-5UG, pkg of vial of cells 89072611-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Fibroblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Application

Growth factors and virus studies: Infectious pancreatic necrotic virus

Biochem/physiol Actions

Goldfish fin tissue

The cells support the growth of grunt fin agent with CPE at 20°C and 25°C. Also susceptible to infectious pancreatic necrosis virus of trout at 28°C.

Preparation Note

BME (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin; 5% CO₂; 25°C. Fish cell lines detach easily during transit if the culture is too young. After resuscitation split cells 1:3 to 1:4 and culture for at least one week with 1-2 media ch

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.