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C9658

Sigma-Aldrich

Carboxypeptidase G from Pseudomonas sp.

lyophilized powder, ≥3 units/mg protein

Synonyma:

γ-Glutamyl hydrolase

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

Číslo CAS:
9074-87-7
Číslo enzymu podle klasifikace EK:
3.4.17.11 (BRENDA, IUBMB)
EC Number:
232-978-1
MDL number:
MFCD00130719
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

300

specific activity

≥3 units/mg protein

mol wt

homodimer ~90 kDa

composition

Protein, ~70% biuret

solubility

H2O: soluble 0.5 mg/mL

storage temp.

−20°C

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Application

Carboxypeptidase G from Pseudomonas sp., or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Biochem/physiol Actions

Carboxypeptidase G is a lysosomal, thiol-dependent protease, which progressively cleaves γ-glutamyl pteroyl poly-γ-glutamate yielding pteroyl-α-glutamate (folic acid) and free glutamate. It is considered highly specific for the γ-glutamyl bond, but not for the C-terminal amino acid of the leaving group. Molecular mass of this homodimer is approximately 90 kDa. The enzyme is activated by Zn2+ ions.

Unit Definition

One unit will hydrolyze 1.0 μmole of L-glutamic acid from (+)amethopterin per min at pH 7.3 at 30 °C.

Physical form

Contains sodium acetate salt.

Preparation Note

Chromatographically purified
Solutions should be prepared fresh prior to use.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

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Current opinion in plant biology, 11(5), 548-553 (2008-08-12)
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Phosphonate and phosphinate analogues of N-acylated gamma-glutamylglutamate were tested for the ability to inhibit glutamate carboxypeptidase II (GCP II). All of the compounds inhibit GCP II with IC(50) values in the low nanomolar range. The comparison of the results to
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