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A6535

Sigma-Aldrich

Apyrase from potatoes

ATPase ≥200 units/mg protein, lyophilized powder

Synonyma:

Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase

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About This Item

Číslo CAS:
9000-95-7
Číslo enzymu podle klasifikace EK:
3.6.1.5 (BRENDA, IUBMB)
EC Number:
232-569-8
MDL number:
MFCD00130542
UNSPSC Code:
12352204
NACRES:
NA.54

type

Grade VII

Quality Level

300

form

lyophilized powder

ATPase activity

≥200 units/mg protein

secondary activity

≥50 % of base activity ADPase

composition

Protein, 25-60%

solubility

H2O: soluble 1.0 mg/mL

application(s)

diagnostic assay manufacturing

foreign activity

Acid Phosphatase ≤2% of base activity

storage temp.

−20°C

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Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation .
At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+Pi → AMP+2Pi.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis . Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Packaging

Sold on the basis of ATPase units.

Other Notes

This grade has a low ATPase/ADPase ratio.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Derived from red potato

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Osvědčení o analýze (COA)

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