A7646

Apyrase from potatoes

ATPase ≥60 units/mg protein, lyophilized powder (partially purified)

• Synonyma: Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• Číslo CAS: 9000-95-7
• Číslo enzymu podle klasifikace EK: 3.6.1.5 (BRENDA, IUBMB)
• EC Number: 232-569-8
• MDL number: MFCD00130542
• UNSPSC Code: 12352204
• NACRES: NA.54

Vlastnosti

• biological source: potato
• type: Grade III
• form: lyophilized powder (partially purified)
• ATPase activity: ≥60 units/mg protein
• mol wt: ~49 kDa by gel filtration
• composition: Protein, 30-60%
• solubility: H₂O: soluble 10 mg/mL, slightly hazy
• application(s): diagnostic assay manufacturing
• foreign activity: Acid Phosphatase ≤5% of base activity
• storage temp.: −20°C

Popis

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1 Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation. The enzyme has been used to assess the possibility of ATP involvement in intestinal lamina propria Th17 cell differentiation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM. The molecular weight of the protein is found to be approximately 45 kDa.

Packaging

Sold on the basis of ATPase units

Other Notes

Mixture of high and low ratio isoenzymes.

Quality

Contains ≤ 5% acid phosphatase.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Bezpečnostní informace

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)