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Merck

R1383

Sigma-Aldrich

Medio RPMI-1640

With ʟ-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

Sinónimos:

Roswell Park Memorial Institute 1640 medium

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Medio RPMI-1640, With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

form

powder

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: yes
phenol red: yes
sodium pyruvate: no
HEPES: no
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

El medio RPMI 1640 lo desarrolló el equipo de Moore en el Instituto Roswell Park Memorial en 1966. Se formuló una modificación del medio McCoy 5A para sostener las células linfoblastoides en cultivo en suspensión, pero desde entonces se ha demostrado que sirve de soporte para una amplia variedad de células que necesitan anclaje. Originalmente indicado para utilizar con un complemento sérico, se ha demostrado que el RPMI 1640 respalda diversas líneas celulares en ausencia de suero. También se ha utilizado ampliamente en protocolos de fusión y en el crecimiento de células híbridas. Este medio es adecuado para el cultivo de leucocitos humanos sanos y neoplásicos.

Application

RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.

Quantity

Formulated to contain 8.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Renée J H A Tillie et al.
Frontiers in cell and developmental biology, 9, 695684-695684 (2021-08-31)
The protein 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 (PFKFB3) is a key stimulator of glycolytic flux. Systemic, partial PFKFB3 inhibition previously decreased total plaque burden and increased plaque stability. However, it is unclear which cell type conferred these positive effects. Myeloid cells play an important
Jiayi Yao et al.
Cancers, 12(8) (2020-08-09)
The acidic pH of the tumor microenvironment plays a critical role in driving cancer development toward a more aggressive phenotype, but the underlying mechanisms are unclear. To this end, phenotypic and genotypic changes induced by adaptation of cancer cells to
Qinxi Ma et al.
mSphere, 4(3) (2019-06-28)
Fatty acids have known antifungal effects and are used in over-the-counter topical treatments. Screening of a collection of gene knockouts in Candida albicans revealed that one strain, carrying a deletion of the transcription factor DAL81, is very susceptible to the
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Mark Y Jeng et al.
The Journal of experimental medicine, 215(1), 51-62 (2017-12-02)
The expansion of CD8+CD28- T cells, a population of terminally differentiated memory T cells, is one of the most consistent immunological changes in humans during aging. CD8+CD28- T cells are highly cytotoxic, and their frequency is linked to many age-related

Protocolos

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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