Saltar al contenido
Merck

Saltar a

C5670

Calcium chloride

anhydrous, BioReagent, suitable for insect cell culture, suitable for plant cell culture, ≥96.0%

Sinónimos:

CaCl2, Calcium dichloride

Iniciar sesión para ver los precios por organización y contrato.

Seleccione un Tamaño

Cambiar Vistas
A ustedes/SKUDisponibilidadPrecio
100 g
Comprobar disponibilidad del carrito
$106.000
500 g
Comprobar disponibilidad del carrito
$413.000

Acerca de este artículo

Fórmula lineal:
CaCl2
Número CAS:
Peso molecular:
110.98
NACRES:
NA.71
PubChem Substance ID:
UNSPSC Code:
12352207
EC Number:
233-140-8
MDL number:
Assay:
≥96.0%
Grade:
anhydrous
Form:
powder

$106.000


Comprobar disponibilidad del carrito

Solicite una orden a granel
Servicio técnico
¿Necesita ayuda? Nuestro equipo de científicos experimentados está aquí para ayudarle.
Permítanos ayudarle


grade

anhydrous

Quality Segment

vapor pressure

0.01 mmHg ( 20 °C)

product line

BioReagent

assay

≥96.0%

form

powder

technique(s)

cell culture | insect: suitable, cell culture | plant: suitable

mp

772 °C (lit.)

application(s)

advanced drug delivery

SMILES string

[Cl-].[Cl-].[Ca++]

InChI

1S/Ca.2ClH/h;2*1H/q+2;;/p-2

InChI key

UXVMQQNJUSDDNG-UHFFFAOYSA-L

General description

Calcium chloride is a commonly used reagent in biochemistry. Calcium plays important roles in many biological processes, including signal transduction, muscle contraction, and maintenance of cell membrane and cell wall stability.1,2 Extensive reviews of the experimental measurement of biological calcium have been published.3,4Calcium chloride is used in the preparation and transformation of competent E. coli and in the transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA.5 The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied.6 A protocol for the concentration of virus vectors that uses CaCl2 has been published.7CaCl2 has been used in the stabilization and two-dimensional crystallization of the NADH-ubiquinone oxidoreductase (complex I) from Escherichia coli.8 The crystallization of porcine pancreatic elastase in the presence of CaCl2 in the presence of sodium citrate reveals binding of calcium in the metal binding site of the protein.9 The effect of CaCl2 on the total fluorescence in the polymerication of the tubulin-like FtsZ division protein of Escherichia coli has been studied by a multiwell fluorescent assay.10

Application

This product is insect cell culture tested (0.5 mg/ml) and is appropriate for insect cell culture applications. Calcium chloride is used in the preparation and transformation of competent E. coli and in the
transfection of eukaryotic cells with either plasmid DNA or high molecular weight genomic DNA. The CaCl2-mediated electroporation of E. coli with the plasmid DNA pBR322 has been studied. A protocol for the concentration of virus vectors that uses CaCl2 has been published.

Preparation Note

This product is soluble in water (222 mg/ml, 2 M), yielding a clear, colorless solution.

Comparar elementos similares

Ver comparación completa

Mostrar Diferencias

1 of 1

Este artículo
449709793639C1016
form

powder

form

-

form

powder

form

granular

assay

≥96.0%

assay

≥99.9% trace metals basis

assay

≥96%

assay

≥93.0%

Quality Level

200

Quality Level

100

Quality Level

100

Quality Level

200

grade

anhydrous

grade

synthesis grade

grade

ACS reagent, anhydrous

grade

anhydrous

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

vapor pressure

0.01 mmHg ( 20 °C)

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)

mp

772 °C (lit.)


Still not finding the right product?

Explore all of our products under Calcium chloride


pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Eye Irrit. 2

Clase de almacenamiento

13 - Non Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.

Si necesita más asistencia, póngase en contacto con Atención al cliente

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos



Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the procedure for preparing a 2 M solution using calcium chloride and calcium chloride dihydrate?

    1 answer
    1. The molecular weight for calcium chloride dihydrate is 147.01 g/mol, so a 2 M solution would be 294 g/L. Similarly, the molecular weight for calcium chloride is 110.98 g/mole, so a 2 M solution would be 222 g/L. Therefore, a 2 M solution of calcium chloride dihydrate (such as C7902) would be 294 g/L with a molecular weight of 147.01 g/mol.

      Helpful?

Reviews

No rating value

Active Filters