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Merck

L1900

Sigma-Aldrich

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

Sinónimos:

Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified

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About This Item

UNSPSC Code:
41106200
NACRES:
NA.72

Quality Level

form

powder

technique(s)

microbiological culture: suitable

application(s)

agriculture
microbiology

General description

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Components

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Plasmid DNA topology assayed by two-dimensional agarose gel electrophoresis
Schvartzman JB, et al.
Journal of Separation Science, 121-132 (2013)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
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Journal of Separation Science, 26.0, 20-28 (2003)
Facile monitoring and evaluation of bacteria in a fermentation process using perfusion chromatography and polarimetry detection
Berthod A, et al.
Journal of Separation Science, 26.0, 20-28 (2003)

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