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CMC0014

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BL21(DE3) Chemically Competent Cells

Escherichia coli, rod shaped

Sinónimos:

Competent E. coli cells

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About This Item

Código UNSPSC:
41106202
NACRES:
NA.85

Nombre del producto

BL21(DE3) Chemically Competent Cells, for protein expression

origen biológico

Escherichia coli

grado

Molecular Biology
for molecular biology

modo de crecimiento

adherent or suspension

morfología

rod shaped

técnicas

microbiological culture: suitable

transformación celular

competent cell type: chemically competent
transformation efficiency: ≥1 x 107 cfu/μg

Condiciones de envío

dry ice

temp. de almacenamiento

−70°C

Descripción general

The BL21(DE3) Chemically Competent strains contain the phage T7 RNA polymerase gene linked to the IPTG-inducible promoter, for use with any expression plasmid containing the T7 promoter.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Aplicación

The E. coli strain BL21 (DE3) has been used:

  • to express tau 2N4R isoform (aa1–441) and the truncated tau (aa1–421) using pET19b as expression vector.
  • BL21(DE3)Chemically Competent Cells has been used for the transformation and expressionof protegrin-1 (PG1) peptides.
  • to express 6His-TEVsite-Hs ARF1 (17-181)-Q71L in pHis2 vector.

Características y beneficios

  • a transformation efficiency of >1 × 107 cfu/μg.
  • economical prices
  • offering superior value for everyday protein expression work.

Componentes

  • BL21(DE3) chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression

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Recovery Medium for Expression, for use with competent cells

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000346689
SLCM7271
SLCM4785
SLCP0759
SLCN4860

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David B Sauer et al.
eLife, 9 (2020-09-02)
Citrate, α-ketoglutarate and succinate are TCA cycle intermediates that also play essential roles in metabolic signaling and cellular regulation. These di- and tricarboxylates are imported into the cell by the divalent anion sodium symporter (DASS) family of plasma membrane transporters
Yasaman Damestani et al.
Lasers in surgery and medicine, 48(8), 782-789 (2016-10-19)
The development and feasibility of a novel nanocrystalline yttria-stabilized-zirconia (nc-YSZ) cranial implant has been recently established. The purpose of what we now call "window to the brain (WttB)" implant (or platform), is to improve patient care by providing a technique
A validated antibody panel for the characterization of tau post-translational modifications.
Ebru E, et al.
Mol. Neurodegener., 12(1), 87-87 (2017)
Romana Koszagova et al.
Microbial cell factories, 17(1), 139-139 (2018-09-05)
Physiological aggregation of a recombinant enzyme into enzymatically active inclusion bodies could be an excellent strategy to obtain immobilized enzymes for industrial biotransformation processes. However, it is not convenient to recycle "gelatinous masses" of protein inclusion bodies from one reaction
Gints Kalnins et al.
Nature communications, 11(1), 388-388 (2020-01-22)
Bacterial microcompartments (BMCs) are prokaryotic organelles consisting of a protein shell and an encapsulated enzymatic core. BMCs are involved in several biochemical processes, such as choline, glycerol and ethanolamine degradation and carbon fixation. Since non-native enzymes can also be encapsulated
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Contenido relacionado

BL21 Chemically Competent Cells Overview

BL21 Chemically Competent Cells come in aliquots for easy transformation with heat shock method, facilitating molecular biology experiments.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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