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T5574

Sigma-Aldrich

Terrific Broth

Liquid microbial growth medium

Sinónimos:

TB medium, Tartoff – Hobbs medium

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About This Item

Código UNSPSC:
41106200
NACRES:
NA.85

grado

for molecular biology

Nivel de calidad

500

descripción

Liquid Medium

esterilidad

sterile; 0.2 μm filtered

Formulario

liquid

técnicas

microbiological culture: suitable

aplicaciones

microbiology

temp. de almacenamiento

room temp

idoneidad

nonselective for Escherichia coli
nonselective for coliforms

Categorías relacionadas

Descripción general

Terrific Broth is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains glycerol, peptides, amino acids, water-soluble vitamins, and carbohydrates.

Aplicación

Terrific Broth has been used for non-selective cultivation of E. coli strains for cloning, high-yield DNA plasmid production, and high-yield production of recombinant proteins. citation It is also suitable for selective cultivation when appropriate antibiotics are added.

Características y beneficios

Terrific Broth liquid provides:
  • Filter-sterilized, ready-to-use format
  • Convenient package size
  • Standard formulation

Componentes

23.6g/L Yeast Extract
11.8g/L Tryptone
9.4g/L K2HPO4
2.2g/L KH2PO4
4ml/L Glycerol

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 2

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves

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Certificados de análisis (COA)

Lot/Batch Number
0000408192
0000408192
SLCN1739
SLCH8540
SLCG5460

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Shaheen Shojania et al.
Journal of virological methods, 164(1-2), 35-42 (2009-11-28)
The HIV-1 transactivator of transcription (Tat) is a protein essential for virus replication. Tat is an intrinsically disordered RNA-binding protein that, in cooperation with host cell factors cyclin T1 and cyclin-dependent kinase 9, regulates transcription at the level of elongation.
Shani Leviatan et al.
The Journal of biological chemistry, 285(31), 23548-23556 (2010-06-08)
Membrane proteins constitute 20-30% of all proteins encoded by the genome of various organisms. Large amounts of purified proteins are required for activity and crystallization attempts. Thus, there is an unmet need for a heterologous membrane protein overexpression system for
The preparation and analysis of DNA for use in transgenic technology.
Auerbach, A.B., et al.
Advanced Protocols For Animal Transgenesis, 501-501 (2011)
Andrey Sokolov et al.
Proceedings of the National Academy of Sciences of the United States of America, 107(3), 969-974 (2010-01-19)
Whereas the laws of thermodynamics prohibit extraction of useful work from the Brownian motion of particles in equilibrium, these motions can be "rectified" under nonequilibrium conditions, for example, in the presence of asymmetric geometrical obstacles. Here, we describe a class
Ørjan Samuelsen et al.
Antimicrobial agents and chemotherapy, 52(8), 2905-2908 (2008-06-19)
Purified recombinant VIM-7 possesses efficient penicillinase and carbapenemase activities comparable to those of VIM-2. Cephalosporinase activity was variable and generally lower than those of VIM-1 and VIM-2. A homology model suggests that the VIM-7 Tyr-218 Phe substitution may be responsible
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Protocolos

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

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